slation Detection
Transcend and FluoroTect Detection Systems offer: • imple labeling: add 1μl to the translation reaction S • ost savings: no antibody reagents needed C • ensitive detection: equivalent to 35S-methionine S Sensitivity comparison: Luciferase was expressed and labeled using three different labeling techniques.
µg
A.
0
2
1 0.75 0.5 0.25 0.1 0.05 0.01
FluoroTect GreenLys In-gel fluorescence
—61kDa luciferase
Detection down to 50ng!
B.
—30kDa endogenous fluorescent protein 0 2 1 0.75 0.5 0.25 0.1 0.05 0.01
Transcend Chemiluminescent Western blot
µg
—61kDa luciferase
S-methionine µg Visualized overnight with phosphorimaging
35
C.
0
2
10.75
0.5 0.25 0.1 0.05
0.01 —61kDa luciferase
3138TA
13
Pagina 12
Automation
Hands-free Viral Nucleic Acid Extraction with the Maxwell® 16 System
The Maxwell 16 Instrument is a highly versatile instrument for automated purification of DNA, RNA and recombinant proteins. The instrument is preprogrammed with protocols and uses prefilled reagent cartridges to make purification simple and convenient. Combine the Maxwell 16 Instrument with low elution volume (LEV) hardware and the Maxwell Viral Total Nucleic Acid Purification Kit and you can: • solate high quality, nucleic acid I from 1–16 samples in under 1hr • oncentrate nucleic acids to 50μl C • et ready-to-use purified nucleic G acids for qPCR or RT-qPCR
Personal
14
Pagina 14
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