Limiting amounts of sample, difficult sample types, and the potential for carryover of PCR inhibitors into the purified product pose problems for many labs during DNA extraction procedures. Automated sample extraction using Maxwell® 16 overcomes these problems and gives high yields of clean DNA-- even from challenging samples.
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PubHub
Maximize your chance of success when sample size is limiting: The graph below shows the yield obtained from varying numbers of cells using the Maxwell® 16 Cell LEV DNA Purification Kit, and shows that DNA was successfully extracted from as few as 10 cells. See the protocol. Purify DNA from difficult sample types: It can be difficult to consistently obtain quality DNA from certain sample types, e.g., amniotic or cerebrospinal fluid. The prefilled optimized reagent cartridges of the Maxwell® 16 Cell LEV DNA kit offer maximum sensitivity and provide consistent results, even with inherently difficult samples. With Maxwell, you also have access to a database of sample types tested that includes details of preprocessing requirements, yield and downstream applications. Obtain virtually inhibitor-free DNA from FFPE samples: Formalin-fixed, paraffin-embedded tissues can be problematic because of the potential for carryover of inhibitors into the purified DNA. The Table shows yields from FFPE samples using the Maxwell 16 FFPE Tissue LEV DNA Purification Kit, and the Ct values obtained in subsequent qPCR assays. The purified
DNA did not affect amplification of an internal PCR control, indicating that it was inhibitor-free, and also gave clear results in MSI analysis experiments. See the poster. Interested in learning more? Contact us to schedule a demo or quote for your lab.
1,000 100
Jurkat HEK 293 HeLa
Yield (ng)
10 1 0.1 0.01
1
10
100
1,000
10,000
100,000 1,000,000
7853MA
Cell Number
Magnesil® Fixed Tissue
Maxwell® 16 FFPE Tissue
Sample Avg (pg/ml)
IPC (Ct)
Sample Avg (pg/ml)
IPC (Ct)
Tissue A Tissue B Tissue C
37=2 14=8 6=1
20 20 20
49=3 13=8 3=1
20 20 20
Demo or Quote
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Maxwell
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Promega US eNews - February 2010 Lees publicatie 12Home