FuGENE
Get more from your GLO
More Biologically Relevant Low toxicity, less impact on biology Simple Protocol No culture changes, less variability, compatible with serum Effective in Many Cell Types Online database with over 40 cell types, including primary and stem cells Ideal for Use with Luciferase Assays More expression, sensitive result
FuGENE® HD is sold only for research use by nonprofit entities. See terms of use at: www.promega.com/lull. FuGENE is a registered trademark of Fugent, L.L.C., USA.
Maxwell
FuGENE® HD offers superior transfection while maintaining viability
HEK293 cells were transiently transfected with a constitutive luciferase expression vector. Three different lipid-based transfection reagents (A, B and FuGENE® HD) were optimized according to the manufacturers’ instructions. Cell viability (using the CellTiter-Fluor™ Cell Viability Assay; Cat.# G6080) and reporter activity (using the ONE-Glo™ Luciferase Assay System; Cat.# E6110) then were assayed using the GloMax®-Multi Detection System Instrument (Cat.# E7031). Data were generated using optimal conditions for each reagent (conditions that gave the highest reporter activity with relatively minor loss in viability). Data are the average of replicate samples ± SEM. For each optimization, a minimum of 3 lipid reagent:DNA ratios and 2 cell densities were tested.
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