FuGENE® HD offers superior transfection while maintaining viability
HEK293 cells were transiently transfected with a constitutive luciferase expression vector. Three different lipid-based transfection reagents (A, B and FuGENE® HD) were optimized according to the manufacturers’ instructions. Cell viability (using the CellTiter-Fluor™ Cell Viability Assay; Cat.# G6080) and reporter activity (using the ONE-Glo™ Luciferase Assay System; Cat.# E6110) then were assayed using the GloMax®-Multi Detection System Instrument (Cat.# E7031). Data were generated using optimal conditions for each reagent (conditions that gave the highest reporter activity with relatively minor loss in viability). Data are the average of replicate samples ± SEM. For each optimization, a minimum of 3 lipid reagent:DNA ratios and 2 cell densities were tested.
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