of Molecular Tools
human genome saw the development of the entire field of molecular biology and the associated emergence of the fundamental techniques that made the human genome project a viable proposition. Key events in that history of molecular biology include the discovery and mass production of restriction and DNA modifying enzymes, the invention of PCR and qPCR, the development of DNA purification methods, and the invention of automated sequencing. The evolution of various molecular tools and the development of the computing power to assist in data analysis have had a major impact on
the pace of discovery over the years. Many of those key techniques are now a routine part of daily laboratory life. On their own they were significant discoveries, taken together they have transformed life science research.
Learn more about basic molecular biology techniques:
PCR Cloning Transfection DNA Purification PCR Protocols Online The Chemistry of Inheritance Cycle Sequencing Animation Restriction Enzyme Resource
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Evolution
GoScript™ Reverse Transcriptase
delivers robust, reliable cDNA synthesis optimized for qPCR assays
GoScript™ replaces SuperScript® I, II, III and other RTs
• Ultra-Active: Save money on every reaction. • Sensitive: Detect rare transcripts. • Processive: Transcribe long messages. • Resilient: Synthesize cDNA in the presence of strong inhibitors.
40.000 35.000 30.000 25.000 20.000 15.000 10.000 5.000 .001 -0.100
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GoScript™ Reverse Transcriptase cDNA synthesis coupled with GoTaq® qPCR Master Mix can provide detection of input templates over a 9-log order dynamic range.
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GoScript
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Promega US eNews - February 2010 Lees publicatie 12Home