The Promega @cademy provides a tailored programme of non-commercial webinars, covering a wide range of scientific topics, from basic concepts to highly technical research presentations. The Promega @cademy webinars are free of charge, though we do ask you to register upfront. Monday 20 September 2010 4.00 PM - 5.00 PM (CEST)
“Stem Cell Line Authentication and Contamination Detection.” (Specialist Webinar) Information and Registration
Monday 27 September 2010 4.00 PM - 5.00 PM (CEST)
“Monitoring Human Stem Cell Differentiation and Pluripotency via Multiplex qPCR” (Specialist Webinar) Information and Registration
Basic webinars address the fundamentals of specific topics and technologies. They are especially useful for students, technicians and junior scientists. Specialist webinars address more advanced topics and are useful for PhD students and senior scientists.
Visit @cademy
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Immobilized Trypsin Protein Digestion in as little as
Many protein digests are performed in solution to avoid the time-consuming steps associated with in-gel digestion. Trypsin is the enzyme of choice for creating small peptide fragments for mass spectrometry analysis. Trypsin cleaves at the C-terminus of lysine (K) and arginine (R) amino acids, resulting in an Nterminal amine group. The basic side chain residues of K, R and the N-terminus become protonated and are easily ionized when analyzed by mass spectrometry. However, separating trypsin from the peptide fragments requires extra purification steps and can complicate the analysis if residual enzyme is left in the sample.
Immobilized trypsin provides a fast and convenient alternative for digesting proteins and separating the trypsin away from the peptide fragments. Promega has immobilized trypsin on cellulose particles and provided spin columns for easy protein digestion without trypsin contamination. An aliquot of particles is packed in the spin column and after three brief washing/equilibration steps, 20-500µg of protein is added to the column. A variety of protein samples can be digested without the need to concentrate or optimize digestion conditions. After a 30-minute digestion, peptides are easily separated from the immobilized trypsin by
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Promega Benelux eNews - August 2010 Lees publicatie 4Home