3D CELL VIABILITY Measure Cell Viability in 3D Microtissues Using the CellTiter-Glo® 3D Assay Cells in 3D culture provide more physiologically relevant data than cells studied in standard 2D formats. However, most reagents available to analyze cell health are optimized for 2D cell culture and may not be effective for 3D microtissues. The CellTiter-Glo® 3D Assay was designed to measure cell viability of 3D cultured cells by quantitating the amount of ATP, a hallmark of metabolically active cells. This single reagent efficiently lyses and recovers ATP from 3D microtissues grown in a variety of 3D culture models, including extracellular matrix (ECM)-independent (e.g., hanging drop) and ECM-dependent (e.g., Matrigel®). The main drawback to using 2D-optimized assays for 3D microtissues is the inability to access all the cells in the 3D culture. As a result, the data may not fully represent the health of the cells. Figure 1 demonstrates how the luminescent CellTiter-Glo® 3D Assay is more sensitive than alamarBlue® or MTT assays when assessing cell viability in 3D microtissues. Regardless of the method used to culture cells in 3D, the CellTiter-Glo® 3D Assay offers consistent results (Figure 2). Learn More Pagina 3
Pagina 5Voor magazines, online gebruiksaanwijzingen en archief zie het Online Touch content management system systeem. Met de mogelijkheid voor een webwinkel in uw kranten.
Promega Benelux - Monthly online Magazine - April 2015 edition Lees publicatie 100Home