CYTOTOXICITY Gain More Cell Health Data by Multiplexing With the CellTox™ Green Cytotoxicity Assay T he CellTox™ Green Cytotoxicity Assay measures changes in membrane integrity that occur due to cell death. The assay is intended for assessing cytotoxicity in cell culture after experimental manipulation. The assay system uses an asymmetric cyanine dye that is excluded from viable cells but preferentially stains the DNA in dead cells (Figure 1). Viable cells produce no appreciable increase in fluorescence; therefore, the fluorescence signal produced by the binding to dead cell DNA is proportional to cytotoxicity. The dye can be diluted in culture medium and delivered directly to cells at the time of seeding or when compound is added to kinetically measure cytotoxicity (no-step method). The dye can also be diluted in assay buffer and delivered to cells as a conventional endpoint measure. DNA is more stable and therefore gives a steadier, longer lasting signal than other current cytotoxicity chemistries. enews | April 2015 Pagina 7
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Promega Benelux - Monthly online Magazine - April 2015 edition Lees publicatie 100Home