scent Assays Detect NAD+, NADP+, NADH and NADPH Linear Range and Specificity 1 × 106 2 × 106 3 × 106 4 × 106 5 × 106 6 × 106 7 × 106 A. NAD+ NADH NADP+ NADPH NAD/NADH-Glo™ can be used to monitor cellular or biochemical levels of total oxidized and reduced nicotine adenine dinucleotide. It can also be used to determine NAD+/NADH ratios. 8 × 10 6 × 105 4 × 105 2 × 105 0 8 × 105 6 × 10 4 × 10 2 × 10 0050 100 150 200 250 300 350 400 [Dinucleotide], nM 0102030440 0 10 20 30 6 × 106 5 × 106 4 × 106 3 × 106 2 × 106 1 × 106 B. 0 1 × 105 2 × 105 3 × 105 4 × 105 5 × 105 0 0 0110 050 100 150 200 250 300 350 400 [Dinucleotide], nM NADH, NADPH, NAD+ and NADP+ stocks were prepared and diluted to the indicated concentrations in phosphatebuffered saline. Fifty-microliter samples at each dinucleotide concentration were incubated with 50μl of NAD/NADH-Glo™ (Panel A) or NADP/NADPH-Glo™ (Panel B) Detection Reagent in white 96-well luminometer plates. After a 30-minute incubation, luminescence was measured. The limit of detection was approximately 1nM for NAD/ NADH-Glo™ and 0.5nM for NADP/NADPH-Glo™. 0220 0330 0440 NADP/NADPH-Glo™ can be used to monitor cellular or biochemical levels of total oxidized and reduced nicotine adenine dinucleotide phosphates. It can also be used to determine NADP+/ NADPH ratios. NADP+ NADPH NAD+ NADH NAD(P)H-Glo™ can be used in biochemical screening to measure the reduced forms of nicotine adenine dinucleotides. It does not discriminate between NADH and NADPH. Ordering Information NAD/NADH-Glo™ Assay NADP/NAPHGlo™ Assay NAD(P) H-Glo™ Assay enews | September 2013 Luminescence (RLU) Luminescence (RLU) 11730MA 11731MA Pagina 4
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Promega Australia - Monthly online Magazine - September 2013 edition Lees publicatie 10Home