Energy transfer from protein interactions results in fluorescence. Protein A Protein B When proteins tagged with NanoLuc® Luciferase and NanoLuc® + substrate Fluorescence HL: HaloTag® NanoBRET™ 618 Ligand HT: HaloTag® protein HL O O HT HaloTag interact, it causes an energy transfer that results in fluorescence emission. NanoBRET™ Assays offer you THREE purchasing options: Buy a Prebuilt Assay: Choose from a large selection of optimized NanoBRET™ Assays for many key protein:protein interactions— including epigenetic assays to study bromodomain/histone interactions in the context of native chromatin. Assays are available by direct order from our catalog or by custom order. Build Your Own Assay: Vectors to create your own NanoLuc® and HaloTag® fusion proteins plus reagents and controls to get started. Order a Custom Assay: Can’t find the protein interactions assay you want? Let us build it for you. Learn More about NanoBRET™ Options » enews | July 2015 Pagina 12
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