PROTEIN DEAMIDATION Sensitively Quantitate Protein Deamidation NH The ISOQUANT® Isoaspartate Detection Kit is intended for quantitative detection of isoaspartic acid residues in proteins and peptides, which can result from the gradual, nonenzymatic deamidation of asparagine or rearrangement of aspartic acid residues during storage or handling. Because the kit does not depend on the monitoring of charge differences for detection, charge heterogeneity does not interfere with the assay. The ISOQUANT® Kit can be used on peptides or proteins such as monoclonal antibodies. ✔ Economical: Detection by HPLC eliminates cost and inconvenience of handling radioactive materials. ✔ Versatile: Assay samples individually or in batches. Small sample size makes the assay suitable for research, analytical methods, formulations and process development work. ✔ Robust: Detection levels as low as 10pmol. Not affected by common buffer components. H2C CH O C O– isoaspartic acid site PIMT H2C NH CH O C O C O NH CH R methylated intermediate *CH3 H2C CH C O N NH CCH O R succinimide intermediate methanol + Detection of isoaspartic acid via PIMT-catalyzed generation of SAH and methanol. Ordering Info © 2015 Promega Corporation. GloMax, GoTaq, ISOQUANT, Maxwell and QuantiFluor are registered trademarks of Promega Corporation. BacTiter-Glo and ReliaPrep are trademarks of Promega Corporation. Nanovue is a trademark of GE Healthcare Bio-sciences. Products may be covered by pending or issued patents or may have certain limitations. Please visit www.promega.com for more information. Contact us enews | April 2015 O C SAM SAH O C C O NH CH R C O Pagina 9
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