Cq 11724MA Probe-Based qPCR/RT-qPCR GoTaq® Probe qPCR and RT-qPCR Systems are ready-to-use 2X master mixes optimized for quantitative PCR in the hydrolysis probe detection format. The 1-Step and 2-Step RT-qPCR Master Mixes contain GoScript™ Reverse Transcriptase for efficient first-strand cDNA synthesis. This 2X master mix contains all required components; just add probe, primer and template. GoTaq® Probe qPCR Master Mix allows probe-based real-time qPCR with high reproducibility and sensitivity. It is resistant to PCR inhibitors making it a highly efficient product for gene expression analysis. Comparison: Standard vs. FAST Cycling Earlier Cq Values 10 15 20 25 30 35 40 0 5 Promega—Standard 19.3 Promega—FAST Vendor L—FAST 19.2 20.9 100ng 10ng 21.7 22.2 23.1 1ng 24.3 24.7 25.4 0.1ng 10pg 26.3 26.9 27.8 29.0 29.3 30.3 1pg 31.1 31.7 33.2 0.1pg 33.8 34.9 35.5 Figure 2. GoTaq® Probe qPCR Master Mix gives comparable results when GAPDH is amplified from human pancreas cDNA using standard and fast thermal cycling conditions. Earlier Cq values were observed for both standard and fast thermal cycling conditions when GoTaq® Probe qPCR Master Mix was compared to a competing product (Vendor L). Dye- or probe-based qPCR/RT-qPCR: Learn More » enews | September 2015 Dye or Probe Based QPCR
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Promega eNews September 2015 - Benelux Lees publicatie 10925Home