The benefits of digestion with Trypsin/Lys-C Mix: • Increased peptide and protein identifications due to improved digestion • Improved reproducibility • Tolerance to trypsin-inhibiting contaminants Trypsin/Lys-C Mix Increases Peptide Identification SSGIAEFWIN GTPLVKKGLR QGYFVEAQPK IVLGQEQDSY GGKFDRSQSF VGEIGDLYMW DSVLPPENIL SAYQGTPLPA NILDWQALNY EIRGYVIIKP LVWV 1.7–4.6 increase in peptide peak area Trypsin digest 2207 3743 Peptide Peak Area Trypsin/Lys-C digest Trypsin digest 555 1180 Peptide Peak Area Incomplete digestion with trypsin was evident after comparing peptide peak intensities in trypsin and Trypsin/Lys-C Mix digests. Trypsin/Lys-C Mix Improves Digest Reproducibility 20% Low CV in Trypsin/Lys-C digest 10% Trypsin digest (n=10) Trypsin/Lys-C digest (n=10) 0% ALPAPIEK SLSLSPGK Peptide Sequences Improved reproducibility of IgG (Rituximab) digestion with Trypsin/Lys-C Mix. Learn More » View a Poster » enews | March 2016 QVQLQQPGAELVK Trypsin/Lys-C digest CV peptide peak area (n=10) Pagina 10
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Promega Australia - Monthly Online Magazine - March 2016 Lees publicatie 10927Home