NANOBIT™ NanoBiT Complementation Assay NanoLuc® Binary Technology (NanoBiT™) is a structural complementation reporter designed for protein:protein interaction (PPI) studies in live cells. NanoBiT has two small subunits optimized for stability, low self-affinity and bright luminescence. The small subunit size (LgBiT, 18kDa and SmBiT, 11 amino acids) minimizes interference with normal protein function, while the bright NanoLuc® signal accommodates low, native expression levels. NanoBiT™ subunit interactions are reversible and can detect rapidly associating and dissociating proteins in live cells. Use NanoBiT in live cells for accurate, real-time monitoring of protein dynamics within a natural cellular context. • Accurately model PPI biology: Small, low affinity tags minimize interference with native protein function; bright signal makes typically low native expression levels detectable. • Monitor protein interactions in live cells: Detect PPI induction and inhibition with real-time kinetics. • Scalable and easy to implement: Use at bench scale or with up to 1,536-well assay formats. Read with most luminometers; no special filters or injectors needed. ™ Pagina 1
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