AUTOMATED RNA PURIFICATION DUAL-REPORTER ASSAYS Extracting High-Quality RNA from Peripheral Blood and Bone Marrow Samples Using an Automated Purification System R NA purification is one of the most critical steps in assays used to monitor changing expression (both qualitative and quantitative) within blood cells. Isolation of RNA is complicated by the fact that RNA is significantly more labile than DNA, making RNA more challenging to purify. Moreover, the use of low-quality RNA may compromise the results of downstream applications, which can cause expensive and time-consuming assay repeats. Many available and reliable RNA purification methods are difficult to implement and labor-intensive due to the complexity of the manual isolation procedures. Here we describe a method to improve the quality and the reproducibility of RNA isolation and to identify an automated method that is simple and risk-free for operators, using the Maxwell® 16 LEV simplyRNA Blood Kit (Cat.# AS1310) for RNA purification from peripheral blood or bone marrow samples on the automated Maxwell® 16 Instrument (Cat.# AS2000). For Research Use Only. Not for use in diagnostic procedures. Pagina 11
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Promega Italy - Online Magazine - Spring 2016 edition Lees publicatie 1Home