BISULFITE CONVERSION Our MethylEdge™ Bisulfite Conversion Kit Tops the Competition (Article Review) E pigenetics refers to heritable changes in gene expression that arise from changes in chromosomes without alteration of DNA sequence. These changes occur throughout all stages of development or in response to environmental factors such as exposure to toxins or chronic stress and are implicated in diseases such as cancer. Epigenetic mechanisms of gene regulation include methylation of DNA (i.e., 5-methylcytidine within CpG dinucleotides). Bisulfite treatment is the gold standard for determining DNA methylation status, and works by converting unmethylated cytosines to uracils which become thymines when subsequently amplified by PCR whereas methylated cytosines remain unchanged. Determining methylation status at the nucleotide level is performed by sequencing after PCR amplification. For Research Use Only. Not for use in diagnostic procedures. Pagina 15
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