Bright NanoBiT™ Signal Results in Greater Sensitivity Compared to Split Firefly Luciferase. A. 4×107 Split Fluc at 21˚C 3×107 NanoBiT Split Firefly 2×107 50,000 100,000 150,000 200,000 250,000 0 1×107 –12 –11 –10 –9 –8 –7 –6 log[Rapamycin] (M) 0 –12 –11 –10 –9 –8 log[Rapamycin] (M) Monitor Protein Interactions Reversibly Isoproterenol cAMP 100 75 50 25 Propranolol cAMP Forskolin cAMP NanoBiT GloSensor cAMP 22F 1000 100 10 1 00 0204060 Time (minutes) Explore More » Figure 2. The NanoBiT™ PRKACA:PRKAR2 interaction is readily reversible, showing rapid dissociation in response to increased cAMP isoproterenol or forskolin), and rapid association in response to decreased cAMP (propranolol). The GloSensor™ cAMP Assay monitors changes in intracellular cAMP independently, demonstrating an inverse correlation to the PRKACA:PRKAR2 interaction. –7 –6 NanoBiT vs. Split Fluc at 21˚C How NanoBiT Works Watch the Video » enews | Spring 2016 GloSensor cAMP 22F (fold signal increase) NanoBiT Luminescence (RLU) (% signal decrease) 13380MA Luminescence (RLU) Pagina 6
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