Cell Signaling 10 20 30 40 50 60 70 80 90 0 Subcloning Tools and Vectors Subcloning Tools Bundle Product Subcloning Tools Bundle Size Cat.# Price (Fr) 1 each M1060 Pls. Enq. For Research Use Only. Not for Use in Diagnostic Procedures. Product may not be available in all countries. Please contact your local representative for more information. Description: Speed your subcloning with these easy-to-use tools. Purchase the Subcloning Tools Bundle, and get LigaFast™ Rapid DNA Ligation System, TSAP Thermosensitive Alkaline Phosphatase, BenchTop 100bp DNA Ladder, Wizard® SV Gel and PCR Clean-Up System and PureYield™ Plasmid Miniprep System for one low price. It’s like getting the PureYield™ Plasmid Miniprep System for free. Features: Amount and Type of Inhibitor Added 0.05 0.1 0.15 0.2 0.25 0 pre-incubation in assay • LigaFast™ Rapid DNA Ligation System: Rapid room temperature ligations of vectors and inserts in as little as 5 minutes. Transform competent bacteria immediately following the reaction. See LigaFast™ Rapid DNA Ligation System Product Information #9PIM822 for protocol. • TSAP Thermosensitive Alkaline Phosphatase: Use rapid protocol (included) to digest and dephosphorylate at the same time or use in standard application. Heat kill the enzyme after the reaction in 15 minutes. Active in common restriction enzyme buffers with no zinc requirement. See TSAP Thermosensitive Alkaline Phosphatase Product Information #9PIM991 for protocol. • BenchTop 100bp DNA Ladder: Ready-to-load marker for agarose gel electrophoresis. Use when gel purifying either vector or insert. • Wizard® SV Gel and PCR Clean-Up System: Rapid gel purification of fragments for 100bp to 10kb. Great for removing enzymes from DNA as well. High-capacity and low elution volume. See Wizard® SV Gel and PCR Clean-Up System Technical Bulletin #TB308 for protocol. • PureYield™ Plasmid Miniprep System: Rapid 10-minute miniprep. Prepare your vector for subcloning or use to screen for recombinants. Go from screening to transfection thanks to the high-quality DNA. See PureYield™ Plasmid Miniprep System Technical Bulletin #TB374 for protocol. Comparison of RNasin® Ribonuclease Inhibitor and Superase•In™ inhibition of RNase A activity. Panel A. Total yeast RNA assay. Total yeast RNA was incubated in the presence of 5ng RNase A for 5 minutes at 37°C in 0.5ml of reaction mix containing 50mM MOPS and 5mM MgCl2 (pH 6.5). The indicated amounts of inhibitor (RNasin® or Superase•In™) were mixed with the RNA prior to RNase addition. After incubation, 0.5ml 10% TCA was added to stop the reaction and to precipitate the large RNA molecules. Storage Conditions: Store the LigaFast™ Rapid DNA Ligation System (M8221) and TSAP Thermosensitive Alkaline Phosphatase (M9910) at –20°C. Store the BenchTop 100bp DNA Ladder at 22–25°C; storage at –20°C can enhance the shelf life of this product. Store the Wizard® SV Gel and PCR Clean-Up System (A9281) and PureYield™ Plasmid Miniprep System (A1223) at 22–25°C. 4 An OD280 measurement was taken of the TCA-soluble material. Panel B. “Pre-incubation” and “in assay” conditions. The total yeast RNA assay was performed as described in Panel A along with an experimental modification of “pre-incubation.” For the pre-incubation assay, the ribonuclease inhibitors were mixed with RNase and incubated for 15 minutes at 22°C. The pre-incubation mix was then added to the RNA. For complete and up-to-date product information visit: www.promega.com/catalog 97 Cloning and DNA Markers RNase A Activity (units) Percent Inhibition of RNase Activity 3109MA10_0A 3074MA09_0A 4u RNasin® 4u Superase•In™ 40u Superase•In™ 400u Superase•In™ RNase A RNase A + 8u RNasin® Ribonuclease Inhibitor 8u Superase•In™ RNase A + 40u Superase•In™ RNase A + Pagina 100
Pagina 102Interactieve digitale brochure, deze nieuwsbrief of onderwijsmagazine is levensecht online geplaatst met Online Touch en bied het van pdf naar online converteren van web catalogussen.
Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home