Science Catalog 2012 Life Cell Signaling DNA Fragment Purification Wizard® SV Gel and PCR Clean-Up System Product Wizard® SV Gel and PCR Clean-Up System Wizard® SV Gel and PCR Clean-Up System and x-tracta™ Gel Extractor Bundle Available Separately Membrane Binding Solution Vacuum Adapters For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Wizard® SV Gel and PCR Clean-Up System is designed to extract and purify DNA fragments of 100bp to 10kb from standard or low-melting agarose gels or to purify products directly from PCR and other common reactions such as restriction digests. Up to 95% recovery is achieved depending upon the DNA fragment size. PCR products are commonly purified to remove excess nucleotides and primers. This membrane-based system, which can bind up to 40μg of DNA, recovers isolated DNA fragments or PCR products in as little as 15 minutes, depending on the number of samples processed. The purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation without further manipulation. Features: • Improved Productivity: Purify DNA fragments or PCR products in as little as 15 minutes. • Enhanced Cloning Results: Up to 95% recovery eluted in as little as 15μl. • Confidence in Results: Purified DNA routinely achieves 700 bases with >98% accuracy in automated fluorescent sequencing. • Applications Tested: DNA is suitable for automated fluorescent sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation without further manipulation. • One System to Do It All: One system can replace up to four kits from other suppliers. Storage Conditions: Store at 22–25°C. Protocol Wizard® SV Gel and PCR Clean-Up System Technical Bulletin Part# TB308 Size Cat.# Price (Fr) 50 preps A9281 126.00 250 preps A9282 536.00 1,000 preps A9285 1928.00 50 preps/25 extractors A9283 Pls. Enq. 250 preps/100 extractors A9284 Pls. Enq. 20 ml A9301 112.00 20 each A1331 45.00 PCR Product % Recovery 9,416 3,199 1,000 500 100 0 50 % Recovery Percent recovery of PCR products (100bp–9kb) from agarose gel slices using the Wizard® SV Gel and PCR Clean-Up System. 2,000 1,800 1,600 1,400 1,200 1,000 800 600 400 200 0 Recombinants 90% Recombinant colonies Nonrecombinant colonies 95% 95% 92% 89% 84% 100 Recombinants 67% Unpurified Purified Purification of PCR products enhances cloning success. A 500bp PCR product was purified with the Wizard® SV Gel and PCR Clean-Up System and cloned into the pGEM®-T Easy Vector. Both the percent recombinants and total number of colonies increase with a pure PCR product. White bars represent recombinant colonies. Blue bars represent nonrecombinant colonies. 106 For complete and up-to-date product information visit: www.promega.com/catalog Size (bp) Total Colonies 4382MA11_3A 4308MB11_3A Pagina 109
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