Science Catalog 2012 Life Cell Signaling MagneSil® Genomic, Large Volume System Product MagneSil® Genomic, Large Volume System Size Cat.# Price (Fr) 8 preps A4080 169.00 48 preps A4082 913.00 96 preps A4085 1735.00 Available Separately eLysis Buffer, Large Volume System For Laboratory Use. 1 L A4091 343.00 Description: The MagneSil® Genomic, Large Volume System, is designed for scalable, automated genomic DNA isolation from large-volume samples, eliminating laborious centrifugation steps and the use of hazardous organic solvents. The system has been automated on the Tecan Freedom EVO® liquid handler, providing walkaway purification of genomic DNA from a variety of starting materials, including 1–10ml whole blood samples, regardless of sample storage or shipping conditions. The instrument uses only the amount of reagents required to process each sample, maximizing efficiency and value per prep. The MagneSil® Genomic, Large Volume System, uses a robust noncentrifugation-based automated method to purify genomic DNA from fresh, frozen or mishandled blood and other samples with similar yields and quality. The system bypasses many of the challenges of traditional centrifugation-based methods by lysing the entire whole blood sample and then directly capturing total genomic DNA from the lysed sample using MagneSil® Paramagnetic Particles (PMPs). The genomic DNA bound to the MagneSil® PMPs is washed to remove contaminants such as heme and cellular proteins, then eluted into an aqueous solution ready for use in downstream applications. There is no need for tedious and lengthy DNA rehydration. The purified genomic DNA is suitable for a variety of downstream applications such as single and multiplex PCR, restriction digestion and real-time PCR. Features: • Improve Productivity: Walkaway automation from blood-collection tube to application-ready DNA. • Rely on an Integrated Solution: One reagent system and automated method provide yield and purity from any sample type (fresh or frozen blood, samples of unknown quality and mixed sample populations). • Enjoy Smart Scalability: Scale sample size from 1–10ml of blood, batch size from 1–96 samples and reagent usage from input sample volume. • Achieve Turnkey Automation: Optimized protocol available for the Tecan Freedom EVO® instrument. This and other validated automated methods are available at: www.promega.com/automethods/ Storage Conditions: Store at 22–25°C. Protocol MagneSil® Genomic, Large Volume System Technical Bulletin Part# TB549 ReadyAmp™ Genomic DNA Purification System Product ReadyAmp™ Genomic DNA Purification System For Research Use Only. Not for Use in Diagnostic Procedures. Description: The ReadyAmp™ Genomic DNA Purification System yields single-stranded DNA (ssDNA) from whole blood or blood stains that may be used directly in amplification reactions without further manipulation. The process takes less than one hour and requires no organic extractions or ethanol precipitations. Features: • Simple and Effective: ReadyAmp™ resin removes PCR inhibitors. • Convenient: Isolated DNA can be used directly in PCR amplifications. Storage Conditions: Store at 22–25°C. Protocol Part# ReadyAmp™ Genomic DNA Purification System Technical Bulletin TB190 Size Cat.# Price (Fr) 100 reactions A7710 242.00 Fixed-Tissue Genomic DNA Purification Product Size Cat.# Price (Fr) MagneSil® Genomic, Fixed Tissue System 100 samples MD1490 536.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The MagneSil® Genomic, Fixed Tissue System provides a fast, simple technique to prepare genomic DNA from formalin-fixed, paraffin-embedded tissue. After an overnight Proteinase K digestion, genomic DNA can be manually purified from formalin-fixed, paraffin-embedded thin tissue sections in less than an hour. Amplifiable genomic DNA can be isolated from 10μm thin sections without centrifugation of the lysate prior to purification. Up to 12 samples can be processed in the manual format using the MagneSphere® Technology Magnetic Separation Stand (twelve-position) (Cat.# Z5342). Features: • Purify High-Quality DNA: The composition of the wash buffers and protocol have been optimized to yield genomic DNA that is largely free of small DNAs, a potent inhibitor of PCR amplification. • Rely on Performance-Tested Amplification Results: Amplify targets in multiplex PCR and targets as large as 450–1,800bp. Storage Conditions: MD1490 consists of two separate items shipped at different temperatures. MD1170 (part 1 of 2 for MD1490 - Processing Module) contains Proteinase K, DTT and Incubation Buffer, which are shipped on dry ice. Store MD1170 at –20°C. MD1180 (part 2 of 2 for MD1490 - Purification Module) contains Lysis Buffer, 2X Wash Buffer, Resin and Elution Buffer, which are shipped at room temperature, 22–25°C. Store MD1180 at room temperature, 22–25°C. Protocol MagneSil® Genomic, Fixed Tissue System Technical Bulletin Part# TB319 114 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 117
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