Cell Signaling Plasmid Purification Wizard® Plus SV Minipreps DNA Purification Systems Product Wizard® Plus SV Minipreps DNA Purification System Wizard® Plus SV Minipreps DNA Purification System + Vacuum Adapters Available Separately Alkaline Protease Solution Vacuum Adapters Size Cat.# Price (Fr) 50 preps A1330 118.00 250 preps A1460 502.00 1,000 preps A1465 1806.00 50 preps A1340 128.00 250 preps A1470 512.00 3 ml A1441 64.00 20 each A1331 45.00 A1465 For Laboratory Use. A1330, A1441, A1331, A1460, A1340, A1470, A6760, A6762, A6764 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Wizard® Plus SV Minipreps DNA Purification System, a silica membrane-based system, provides a simple and reliable method for rapid isolation of plasmid DNA. The entire miniprep procedure can be completed in 45 minutes or less, depending on the number of samples processed. Using the system, plasmid DNA can be purified from 1–10ml of overnight E. coli culture. The purified plasmid DNA can be used directly for automated fluorescent BigDye® terminator DNA sequencing as well as for other standard molecular biology techniques without further manipulation. It also can be used for in vitro transcription reactions when supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511). Features: • Improved Productivity: 20 minipreps processed in less than 45 minutes. • High Performance: 1–20μg of high-quality plasmid DNA, enough for multiple applications. • Safety and Convenience: No phenol extractions or precipitations required. • Flexibility: Choice of spin (microcentrifuge) or vacuum purification formats. • Consistent Quality: Alkaline protease step improves plasmid quality. • Confidence in Results: Purified DNA meets a target of >98% accuracy over 500 bases using pGEM®-3Zf(+) Vector in BigDye® terminator sequencing. Storage Conditions: Store at 22–25°C. Protocol Wizard® Plus SV Minipreps DNA Purification System Technical Bulletin 12 34 56 M kb – 10.0 – 6.0 – 4.0 – 3.0 – 2.0 – 1.5 ––––– 8.0 ––––– 5.0 ––––– 2.5 – 1.0 – 0.75 – 0.50 – 0.25 High-quality restriction digests using plasmid purified with the Wizard® Plus SV Minipreps DNA Purification System. High-copy pGEM®-3Zf(+) Vector (lanes 1–3) and low-copy pALTER®-1 Vector (lanes 4–6) were each digested in two separate Promega restriction enzyme reactions. All lanes show reproducible and efficient cutting of the plasmid DNA. Lane M is the 1kb DNA Ladder (Cat.# G5711). Part# TB225 5 pGEM®-3Zf(+) plasmid sequenced with the T7 Promoter Primer (Cat.# Q5021) using DNA purified with the Wizard® Plus SV Minipreps DNA Purification System. PureYield™ Plasmid Miniprep System Product PureYield™ Plasmid Miniprep System For Research Use Only. Not for Use in Diagnostic Procedures. Description: The PureYield™ Plasmid Miniprep System is designed to rapidly isolate highly pure plasmid DNA. The system provides a rapid method for purification of up to 15μg of plasmid DNA from 600μl to 3ml of bacteria culture. Plasmid DNA can be purified in as little as 10 minutes. The PureYield™ Plasmid Miniprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation (e.g., TNT® Quick Coupled Transcription/Translation System). Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method. The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man® Laboratory Vacuum Manifold). Features: • Improved Productivity: Rapid protocol purifies plasmid DNA in 10 minutes. • Robust Performance: High purity and concentration of plasmid DNA gives proven performance in transfection, cell-free expression and other molecular biology applications. • Confidence in Results: Lysis/neutralization indicator dye ensures success every time. • Flexible: Centrifugation and vacuum protocols are available. Storage Conditions: Store all system components at 22–25°C. Protocol PureYield™ Plasmid Miniprep System Technical Bulletin Part# TB374 Size Cat.# Price (Fr) 100 preps A1223 241.00 250 preps A1222 553.00 For complete and up-to-date product information visit: www.promega.com/catalog 117 DNA and RNA Purification 1612TA09_6A Pagina 120
Pagina 122Heeft u een maandblad, turn pages of online artikelen? Gebruik Online Touch: handleiding van papier naar online converteren.
Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home