Cell Signaling Wizard® MagneSil® Plasmid Purification System Product Wizard® MagneSil® Plasmid Purification System Wizard® MagneSil® Plasmid Purification System, HTP1 Available Separately MagneSil® RED MagneSil® BLUE Cell Resuspension Solution Cell Lysis Solution Neutralization Solution Elution Buffer Collection Plates (4-pack) For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Wizard® MagneSil® Plasmid DNA Purification System provides a simple and reliable method for the rapid isolation of plasmid DNA in a 96-well, high-throughput format. The purified plasmid can be used directly for automated fluorescent sequencing, such as with BigDye® terminator sequencing chemistry, as well as for other standard molecular biology techniques including restriction enzyme digestion. The use of the MagneSil® Paramagnetic Particles for lysate clearing (BLUE) as well as DNA capture (RED) circumvents the need for centrifugation or vacuum manifolds, making the system ideal for full automation on a Beckman Coulter or Tecan instrument. Features: • Improve Productivity: Process multiple plates without user intervention. • Gain Confidence: Consistent performance in fluorescent sequencing reactions. • Automate This Assay: Validated automated methods available at: www.promega.com/automethods/ • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at 22–25°C. Protocol Wizard® MagneSil® Plasmid DNA Purification System Technical Bulletin Part# TB286 100 90 92 94 96 98 2 O.D. 4 O.D. 6 O.D. 8 O.D. 10 O.D. 12 O.D. 160 140 120 100 80 60 40 20 0 Size Cat.# Price (Fr) 4 × 96 preps A1630 870.00 8 × 96 preps A1631 1653.00 100 × 96 preps A1635 Pls. Enq. 100 ml A1641 1660.00 100 ml A2201 1191.00 500 ml A7114 229.00 500 ml A7124 149.00 500 ml A7132 149.00 500 ml A1655 139.00 1 each A9161 19.00 Wizard MagneSil Tfx™ System Product Wizard MagneSil Tfx™ System Available Separately Endotoxin Removal Resin 4/40 Wash Solution For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Wizard MagneSil Tfx™ System provides a simple and reliable method for the rapid isolation of transfection-quality plasmid DNA in a 96-well, high-throughput format. The use of MagneSil® Paramagnetic Particles for lysate clearing as well as DNA capture circumvents the need for centrifugation or vacuum manifolds, allowing DNA purification with the Wizard MagneSil Tfx™ System to be completely automated. An automated method has been developed for use of this product with a Beckman Coulter Biomek® FX robotic workstation. This procedure requires approximately 45 minutes to process a single 96-well plate. The method can be adapted to other robotic workstations, such as the Beckman Coulter Biomek® 2000 or the Tecan Genesis® instrument. Features: • Improve Transfection Results: Use of Endotoxin Removal Resin cuts endotoxin carryover as much as 95% over standard sequencing-grade automated plasmid systems. • Enhance Mammalian Protein Expression: Three- to fivefold increase in protein expression compared to plasmid isolated from an automated sequencing-grade plasmid purification system. • Automate This Assay: Validated automated methods available at: www.promega.com/automethods/ • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at 22–25°C. Protocol Wizard MagneSil Tfx™ System Technical Bulletin Transfection Results Wizard MagneSil Tfx™ Qiagen Turbo Qiagen Ultra Part# TB314 Size Cat.# Price (Fr) 4 × 96 preps A2380 1111.00 100 ml A2191 1230.00 115 ml A2221 402.00 5 3T3 CV-1 HeLa 293s CHO 500 600 Bases Accuracy by read length for plasmid DNA sequenced following isolation using Wizard® MagneSil® Plasmid Purification System. Results demonstrate >700 consecutive bases analyzed with >98% accuracy of base identity. 700 A comparison of transfection efficiencies for different DNA purification systems with various cell lines. A variety of eukaryotic cell lines were transfected with pGL3-Control Vector purified using the Wizard MagneSil Tfx™ System or Qiagen Turbo or Ultra systems. Transfection efficiency was determined by measuring firefly luciferase luminescence, and the results were normalized to those for the Qiagen Ultra system. For complete and up-to-date product information visit: www.promega.com/catalog 123 DNA and RNA Purification Accuracy (%) 2890MA03_0A % of Ultra RLU 3907MA12_2A Pagina 126
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