Science Catalog 2012 Life Cell Signaling PolyATtract® System 1000 Product PolyATtract® System 1000 with Magnetic Stand PolyATtract® System 1000 without Magnetic Stand PolyATtract® System 1000 Magnetic Separation Stand Available Separately PolyATtract® GTC Extraction Buffer Size Cat.# Price (Fr) Scalable Z5420 1043.00 Scalable Z5400 922.00 1 each Z5410 175.00 120 ml Z5531 147.00 Z5420, Z5531, Z5400 For Laboratory Use. Z5410 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The PolyATtract® System 1000 isolates messenger RNA directly from crude cell or tissue lysates, eliminating the need for total RNA isolations. This system uses the MagneSphere® technology for the purification of poly(A)+ RNA, eliminating the need for oligo(dT) cellulose columns. The increased yield of mRNA using this system allows the detection of low-copynumber mRNAs in relatively small amounts of material using Northern blot analysis. The isolated mRNA is suitable for all molecular biology applications, including in vitro translation, cDNA synthesis, PCR analysis, ribonuclease (RNase) protection assays, primer extension and Northern blots. The MagneSphere® Technology Magnetic Separation Stands can be used in conjunction with any of the PolyATtract® Systems and are ideal for applications requiring multiple paramagnetic isolations of biomolecules. Features: • Improved Productivity: mRNA purification directly from tissue or cells in 45 minutes or less. Allows quick collection of magnetic particles. • Flexibility: Works with tissue amounts from 5mg–2g per isolation. Magnetic separation stand (Cat.# Z5410) accommodates 1.5ml, 2ml, 15ml and 50ml tube sizes. • Convenience: No lengthy ethanol precipitation steps, phenol:chloroform extractions, or overnight ultracentrifugation through cesium chloride gradients and lithium chloride (LiCl) precipitations. Storage Conditions: Store at 4°C. Do not freeze the MagneSphere® Paramagnetic Particles. Protocol PolyATtract® System 1000 Technical Manual MagneSphere® Magnetic Separation Products Technical Bulletin PolyATtract® mRNA Isolation Systems Product PolyATtract® mRNA Isolation System I (Refill for Z5200) PolyATtract® mRNA Isolation System II with Magnetic Stand PolyATtract® mRNA Isolation System III with Magnetic Stand PolyATtract® mRNA Isolation System IV (Refill for Z5300) Available Separately Biotinylated Oligo(dT) Probe (50pmol/µl) MagneSphere® Technology Magnetic Separation Stand (two-position) 35 µl Z5261 112.00 1.5 ml Z5332 99.00 12 × 75 mm Z5333 99.00 Z5210, Z5261, Z5200, Z5300, Z5310 For Laboratory Use. Z5332, Z5333 For Research Use Only. Not for Use in Diagnostic Procedures. Size Cat.# Price (Fr) 3 isolations Z5210 299.00 3 isolations Z5200 379.00 15 isolations Z5300 379.00 15 isolations Z5310 299.00 Part# TM228 TB246 Description: Cat.# Z5200 contains sufficient reagents for 3 separate mRNA isolations, each from 1–5mg of total RNA. Cat.# Z5210 contains the same reagents as Cat.# Z5200, excluding the Magnetic Separation Stand. Cat.# Z5300 contains sufficient reagents for 15 separate mRNA isolations, each from 100–1,000μg of total RNA. Cat.# Z5310 contains the same reagents as Cat.# Z5300, excluding the Magnetic Separation Stand. The PolyATtract® mRNA Isolation Systems use the MagneSphere® technology to isolate mRNA rapidly and effectively from total RNA. The systems use a biotinylated oligo(dT) primer to hybridize, at high efficiency in solution, to the 3´ poly(A)+ region present in most mature eukaryotic mRNAs. The hybrids are bound to streptavidin coupled to paramagnetic particles, captured using a magnetic separation stand and washed at high stringency. The mRNA is eluted from the solid phase by the simple addition of ribonuclease-free, deionized water. With total RNA as the starting material, poly(A)+ mRNA is isolated in approximately 45 minutes. The isolated mRNA is suitable for all molecular biology applications, including in vitro translation and cDNA synthesis. Features: • Improved Productivity: Entire mRNA purification process can be completed in approximately 45 minutes. • Highly Pure mRNA: Due to the strength and selectivity of the interaction between streptavidin and biotin, mRNA bound to the biotinylated oligo(dT) is captured by streptavidin-coated magnetic particles. • Confidence in Your Applications: Isolated mRNA is suitable for use with in vitro translation, RT-PCR and cDNA synthesis. • Flexibility: Configurations for use with large or small amounts of cells and tissues. Storage Conditions: Store at 4°C. Do not freeze the MagneSphere® Paramagnetic Particles. Protocol PolyATtract® mRNA Isolation Systems Technical Manual Streptavidin MagneSphere® Paramagnetic Particles Product Streptavidin MagneSphere® Paramagnetic Particles For Laboratory Use. Description: The Streptavidin MagneSphere® Paramagnetic Particles (PMPs) may be used in the magnetic separation or purification of a wide variety of biotinylated nucleic acid or protein molecules. The particles are quality-tested and approved for isolation of both nucleic acids and proteins/antibodies. Features: • Confidence: The Streptavidin MagneSphere® Paramagnetic Particles feature strong, specific binding to biotinylated molecules. • Improved Purity: Enable binding, washing and magnetic separation from undesired materials in a solution. • Flexibility: Applications include purification of DNA, mRNA and proteins. Storage Conditions: Store at 4°C. Do not freeze the paramagnetic particles. Protocol MagneSphere® Magnetic Separation Products Technical Bulletin Part# TB246 Size Conc. Cat.# Price (Fr) 9 ml 1 mg/ml Z5481 284.00 25 ml 1 mg/ml Z5482 750.00 Part# TM021 128 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 131
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