Cell Signaling DNA Quantitation QuantiFluor™ dsDNA System Product QuantiFluor™ dsDNA System For Research Use Only. Not for Use in Diagnostic Procedures. Description: The QuantiFluor™ dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The quantitation of dsDNA is a very important step in many biological applications, particularly in standard molecular biology techniques. The dye shows minimal binding to single stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA. Features: • Specificity: Highly specific to dsDNA, minimal binding to ssDNA, RNA, protein and interfering compounds. • Sensitivity: Significantly increased sensitivity over absorbance at 260nm (NanoDrop® spectrophotometer) for low-concentration samples. Performs better or equal to PicoGreen® dye, and has a detection limit of 50pg/ml. • Ease of Use: System includes all required reagents to quickly set up and quantitate dsDNA. • Instrument Compatibility: Easy to set up on both the QuantiFluor™ and GloMax®-Multi Instruments. Also can be used on the Qubit™ instrument. Storage Conditions: Store at 4°C. Protocol QuantiFluor™ dsDNA System Technical Manual Part# TM346 Fluorescence Quenching Schematic diagram illustrating the Plexor® real-time PCR process. iso-dGTP Dabcyl Size Cat.# Price (Fr) 1 ml E2670 405.00 Plexor® HY System Product Plexor® HY System Size Cat.# Price (Fr) 200 reactions DC1001 1154.00 800 reactions DC1000 4039.00 DW0991 For Laboratory Use. DC1001, DC1500, DC1000 Not For Medical Diagnostic Use. For additional information see page XX. Fluorescent Reporter iso-dC Taq Primer Annealing and Extension Taq Incorporation of Dabcyl-iso-dGTP 5 Autosomal amplification curves and melt curves from a Plexor® HY amplification. For complete and up-to-date product information visit: www.promega.com/catalog 131 DNA and RNA Purification 6826TB 4909MA Pagina 134

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