Cell Signaling PDE-Glo™ Phosphodiesterase Assay Product PDE-Glo™ Phosphodiesterase Assay For Research Use Only. Not for Use in Diagnostic Procedures. Description: The PDE-Glo™ Phosphodiesterase Assay is a luminescent, high-throughput screening (HTS) method for measuring cyclic nucleotide phosphodiesterase activity from purified sources. Cyclic nucleotide phosphodiesterases (PDEs) are involved in a myriad of cellular processes due to their ability to hydrolyze, and thus control, the levels of the second-messenger signaling molecules cAMP and cGMP. The availability of selective inhibitors for PDEs has facilitated their use as tools to study cyclic nucleotide signaling and paved the way to investigate the role of PDEs in cellular and tissue pathologies. The PDE-Glo™ Phosphodiesterase Assay allows lead candidates to be identified from compound libraries. The assay is designed for 384-well plates, but assay volumes can easily be scaled for 96- or 1536-well plates. The PDE-Glo™ Phosphodiesterase Assay is optimized to work with both cAMP- and cGMP-dependent phosphodiesterases. The total time required for the assay from start to finish is less than 1 hour after the PDE reaction is complete. Features: • Versatile: Works with both cAMP and cGMP PDEs. • Sensitive: Excellent signal:background ratios. Scalable to 1536-well plate formats. • Fast and Easy to Use: Assay can be completed in <1 hour. Homogeneous. • Proven Luminescent Technology: Powered by Ultra-Glo™ Luciferase. Non-radioactive. • No Interference by Fluorescent Compounds. Storage Conditions: Store the system at –20°C. See the product label for the expiration date. Protocol PDE-Glo™ Phosphodiesterase Assay Technical Bulletin Part# TB353 Size Cat.# Price (Fr) 1,000 assays V1361 761.00 10,000 assays V1362 Pls. Enq. Phosphorylated protein kinase A substrate ADP P cNMP RR C C ATP luciferin + O2 luciferase Active protein kinase A Protein kinase A substrate oxyluciferin + AMP+ Light cNMP = cAMP or cGMP NMP = AMP or GMP The PDE-Glo™ Phosphodiesterase Assay. cNMP RR CC Inactive protein kinase A holoenzyme cNMP NMP 6 PDE For complete and up-to-date product information visit: www.promega.com/catalog 139 Drug Discovery 6289MA Pagina 142
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