Science Catalog 2012 Life Cell Signaling Sample Preparation DNA IQ™ System Product DNA IQ™ System Casework Extraction Kit Tissue and Hair Extraction Kit (for use with DNA IQ™) Available Separately Lysis Buffer 2X Wash Buffer Elution Buffer DNA IQ™ Resin DNA IQ™ Spin Baskets Microtubes, 1.5ml Proteinase K DTT, Molecular Grade (Dry Powder) Description: The DNA IQ™ System is a DNA isolation system designed specifically for forensic and paternity laboratories. This system employs novel paramagnetic particles to isolate clean DNA for use with short tandem repeat (STR) analysis. The DNA IQ™ System can be used to extract DNA from a variety of sample types, including stains and liquid samples. Protocols for database samples and casework samples are available. The unique DNA IQ™ Resin removes PCR inhibitors and contaminants frequently encountered in casework samples. When working with larger sample volumes, such as those found in paternity and databasing, the DNA IQ™ System can deliver a consistent amount of total DNA. Samples including buccal swabs, liquid blood and stains on FTA® and other blood cards have been used with the DNA IQ™ System. Some samples, such as tissue and hair, require pretreatment with proteinase K. In addition, extracting DNA can be difficult from substrates such as denim, envelopes, carpet, etc. The Casework Extraction Kit increases DNA yield from challenging casework samples and substrates. The kit uses Proteinase K and other reagents to remove proteins and other components from the DNA, helping to ensure maximal yield and recovery of DNA from casework samples. The DNA then can be purified using the DNA IQ™ System. The DNA IQ™ System has been tested with the Plexor® HY System and PowerPlex® Systems to ensure a streamlined process. This translates into reliable products that give optimal results from isolation to quantitation and STR analysis. Genomic DNA isolation using the DNA IQ™ System has been automated on the Biomek® 2000 and 3000 laboratory automation workstations, as well as the Tecan Freedom EVO® liquid handler. Please contact Promega Technical Services for additional information. Features: • Rapid: Only a few quick steps to obtain clean DNA with fewer PCR inhibitors. • Flexible: One simple system for use with casework, paternity and database samples. • Efficient: Sensitive to minute sample sizes. In addition, no harmful organic solvents such as phenol and chloroform are used, so use of a hood is not required and disposal of hazardous chemicals is eliminated. Storage Conditions: Store the DNA IQ™ System at 22–25°C. Store the Tissue and Hair Extraction Kit (for use with DNA IQ™) at –20°C. Store the Casework Extraction Kit at 15–30°C. Size Cat.# Price (Fr) 100 reactions DC6701 468.00 400 reactions DC6700 1638.00 100 reactions DC6745 Pls. Enq. 100 reactions DC6740 347.00 150 ml A8261 375.00 70 ml A8271 72.00 50 ml A8281 72.00 50 ml A8251 10563.00 1,000 /bag V1221 376.00 1,000 /bag V1231 122.00 100 mg V3021 145.00 5 g V3151 133.00 DC6701, DC6700, DC6740, V1231, V3021, V3151 For Research Use Only. Not for Use in Diagnostic Procedures. A8261, A8271, A8281, A8251, V1221 Not For Medical Diagnostic Use. Protocol DNA IQ™ System—Small Sample Casework Technical Bulletin DNA IQ™ System—Database Protocol Technical Bulletin DNA IQ™ Casework Pro Kit for Maxwell® 16 Technical Manual Tissue and Hair Extraction Kit (for use with DNA IQ™) Technical Bulletin Differex™ System Product Differex™ System Manual Differex™ Magnet Available Separately Differex™ Digestion Buffer Differex™ Separation Solution Size Cat.# Price (Fr) 50 samples DC6801 403.00 200 samples DC6800 1411.00 1 each V1591 Pls. Enq. 150 ml A8501 656.00 40 ml A8511 700.00 V1591 For Research Use Only. Not for Use in Diagnostic Procedures. A8501, DC6801, A8511, DC6800 Not For Medical Diagnostic Use. Description: The Differex™ System extracts DNA from sexual assault samples easily and quickly. It provides a simple and fast method for separating male and female fractions of a sample, making it possible to analyze samples more quickly and efficiently. The Differex™ System offers recovery similar to that of the standard method commonly used for differential extraction. The Differex™ System is used in combination with the DNA IQ™ System and Slicprep™ 96 Device on robotic platforms to process up to 48 differential extractions in less than 5 hours, including incubation time, and less than 1 hour of hands-on time. Automated Differex™ System methods are available for the Biomek® 2000 and 3000 laboratory automation workstations, as well as the Tecan Freedom EVO® liquid handler. Please contact Promega Technical Services for additional information. A manual protocol for the Differex™ System is available for laboratories not yet using robotic platforms for DNA extraction. Features: • Automated Differential Extractions: The Differex™ System is the first and only system that allows a forensic laboratory to automate every step of differential extraction. • Direct Compatibility with the DNA IQ™ System and Downstream STR Applications: Clean DNA extracts mean you can be confident in your ability to obtain results regardless of your choice of STR systems. • Robust Results With Even Tough Samples: The Differex™ System works with challenging new and old samples typical of those from sexual assaults. Storage Conditions: Store at room temperature. Protocol Differex™ System Technical Bulletin Differex™ System—For Use With the Differex™ Magnet Technical Manual Part# TBD020 TM331 Part# TB296 TB297 TM332 TB307 Manual Differex™ Magnet. 148 For complete and up-to-date product information visit: www.promega.com/catalog 8650TA Pagina 151
Pagina 153Interactieve e-brochure, deze weekblad of pdf is levensecht online geplaatst met Online Touch en bied het online publiceren van digi-reisgidsen.
Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home