Science Catalog 2012 Life Cell Signaling ViviRen™ In Vivo Renilla Luciferase Substrate Product ViviRen™ In Vivo Renilla Luciferase Substrate For Research Use Only. Not for Use in Diagnostic Procedures. Description: ViviRen™ in vivo Renilla Luciferase Substrate is a uniquely engineered coelenterazine-based compound with protected oxidation sites. These modifications are designed to minimize substrate degradation and autoluminescence. It is reported that the ViviRen™ Substrate demonstrates brighter output when compared to the native coelenterazine substrate when used in an in vivo imaging application in a mouse model. Cat.# P1231 is supplied as a liquid, 60mM in DMSO. Cat.# P1232 is supplied as a lyophilized solid. VivoGlo™ In Vivo Imaging Substrates are provided in cooperation with Xenogen Corporation and Caliper Life Sciences for use in in vivo bioluminescence imaging applications. Features: • Highest Quality Substrates: Eliminate potential interference in assays due to the presence of endotoxins. • Assured Product Integrity: Most products are packaged in amber vials with septa to ensure product integrity as well as offer ease of dilution and use for imaging experiments. Product is packaged with fine tolerances to minimize the need to weigh substrates. • Flexibility and Convenience: Available in multiple sizes to accommodate a variety of experimental settings. Storage Conditions: Store at –20°C. Size Cat.# Price (Fr) 0.37 mg P1231 216.00 3.7 mg P1232 1620.00 pGL4 in vivo Imaging Vectors Product pGL4.50[luc2/CMV/Hygro] Vector pGL4.51[luc2/CMV/Neo] Vector For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pGL4 Luciferase Reporter Vectors are the next generation of reporter gene vectors optimized for expression in mammalian cells. Numerous configurations of pGL4 Vectors are available, including those with the synthetic firefly luc2 (Photinus pyralis) and Renilla hRluc (Renilla reniformis) genes, which have been codon optimized for more efficient expression in mammalian cells. Furthermore, both the reporter genes and the vector backbone, including the ampicillin (Ampr) gene and mammalian selectable marker genes for hygromycin (Hygr), neomycin (Neor) and puromycin (Puror), have been engineered to reduce the number of consensus transcription factor binding sites, reducing background and the risk of anomalous transcription. The pGL4 Vector backbone is provided with either the luc2 or hRluc gene and, in certain vectors, one or both of two Rapid Response™ Reporter genes. The proteins encoded by these Rapid Response™ Luciferase genes respond more quickly and with greater magnitude to changes in transcriptional activity than their more stable counterparts. Vectors are available with a minimal promoter upstream of the lucP reporter gene, useful for cloning in a response element of choice. Vectors also are available with a minimal promoter plus choice of response elements or other promoter elements that can be used to study cellular signaling and other events. These vectors can be used in transient transfections or to generate a stable cell line. All vectors use a destabilized luc2P firefly luciferase gene, resulting in low backgrounds and high levels of induction. Stable cell selection is possible using hygromycin resistance. Features: Improved Sensitivity and Biological Relevance Due to: • Increased Reporter Gene Expression: Codon optimization of synthetic genes for mammalian expression. • Reduced Background and Risk of Expression Artifacts: Removal of cryptic DNA regulatory elements and transcription factor binding sites. • Improved Temporal Response: Rapid Response™ technology available using destabilized luciferase genes. Additional Advantages Include: • Flexible Detection Options: Choice of either synthetic luc2 (Photinus pyralis) or hRluc (Renilla reniformis) reporter genes. • Easy Transition from Transient to Stable Cells: Choice of mammalian selectable markers. • Easy Transfer from Vector to Vector: Common multiple cloning site and a unique SfiI transfer scheme. Storage Conditions: Store at –20°C. Protocol pGL4.50[luc2/CMV/Hygro] Vector Product Information pGL4.51[luc2/CMV/Neo] Vector Product Information Part# 9PIE131 9PIE132 Size Cat.# Price (Fr) 20 µg E1310 612.00 20 µg E1320 612.00 178 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 181
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