Cell Signaling Microbial Detection and Quantitation BacTiter-Glo™ Microbial Cell Viability Assay Product BacTiter-Glo™ Microbial Cell Viability Assay Size Cat.# Price (Fr) 10 ml G8230 175.00 10 × 10 ml G8231 788.00 100 ml G8232 710.00 10 × 100 ml G8233 Pls. Enq. For Laboratory Use. Description: The BacTiter-Glo™ Microbial Cell Viability Assay is a homogeneous method for determining the number of viable microbial cells in culture based on quantitation of the ATP present. ATP is an indicator of metabolically active cells. The homogeneous assay procedure involves adding a single reagent (BacTiter-Glo™ Reagent) directly to bacterial cells cultured in medium and measuring luminescence. The homogeneous format reduces pipetting errors that may be introduced during the multiple steps required by other methods of ATP measurement. The formulation of the reagent supports bacterial cell lysis and generation of a luminescent signal in a homogeneous “add-mix-measure” format. The luminescent signal is proportional to the amount of ATP present, which is directly proportional to the number of viable cells in culture. The assay relies on the properties of a proprietary thermostable luciferase (Ultra-Glo™ Recombinant Luciferase) and a proprietary buffer formulation for extracting ATP from bacteria. The assay has been shown to detect a variety of bacteria and fungi. Features: • Simplify Microbial Detection: The “add-mix-measure” format reduces the number of handling steps to fewer than that required for similar ATP assays, with no separate lysis step, and no injectors required, allowing easy automation. • Get Results Quickly: Data can be recorded in 5 minutes or less after adding reagent and mixing. Superior sensitivity allows you to detect growth or toxicity quickly after inoculation. • Increase Your Sensitivity: Measure ATP from as few as 10 bacterial cells, 1,000-fold more sensitive than absorbance (O.D.) readings. • Choose Your Format: Can be used with various multiwell-plate or single-use formats. Data can be recorded by luminometer or CCD camera. • Process Plates Consecutively: The “glow-type” luminescent signal is stable, with a half-life generally over 30 minutes. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: For long-term storage, the lyophilized BacTiter-Glo™ Substrate and BacTiter-Glo™ Buffer should be stored at –20°C. Protocol BacTiter-Glo™ Microbial Cell Viability Assay Technical Bulletin Part# TB337 BacTiter-Glo™ Reagent 9 Mixer Luminometer Diagram of the BacTiter-Glo™ Microbial Cell Viability Assay protocol. 102 103 104 105 106 107 108 109 10 BacTiter-Glo™ Assay 0.01 0.10 1.00 10.00 05 10 15 20 25 Time (hours) 05 10 15 20 25 Time (hours) Evaluate bacterial growth immediately after inoculation using the BacTiter-Glo™ Assay. When measuring growth by O.D., the first significant measurement (0.25 O.D. with E. coli) did not occur until 5 hours after inoculation. For complete and up-to-date product information visit: www.promega.com/catalog 181 Industrial and Environmental Monitoring Luminescence (RLU) O.D. 600 4609MB 4616MC Pagina 184
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