Cell Signaling Maxwell® 16 System RNA Purification Kits Product Maxwell® 16 LEV simplyRNA Cells Kit Maxwell® 16 LEV simplyRNA Tissue Kit Maxwell® 16 Total RNA Purification Kit Maxwell® 16 Tissue LEV Total RNA Purification Kit Maxwell® 16 Viral Total Nucleic Acid Purification System Available Separately Maxwell® 16 High Strength LEV Magnetic Rod and Plunger Bar Adaptor 1 each SP1070 1200.00 AS1270, AS1280, AS1050, AS1220, AS1225 For Laboratory Use. SP1070 For Research Use Only. Not for Use in Diagnostic Procedures. Cat.# AS1155 CE-marked IVD medical device compliant with EU Directive 98/79/EC on in vitro diagnostic medical devices. This product is only available in certain European countries. Description: The Maxwell® 16 LEV simplyRNA Cells Kit and the Maxwell® 16 LEV simplyRNA Tissue Kit are for use with the Maxwell® 16 Instrument configured with the LEV High Strength Magnetic Rod and Plunger Bar Adaptor. This RNA purification procedure is a simple method with minimal lysate handling before automated purification on the Maxwell® 16 Instrument. The low elution volume is used to generate concentrated high-quality RNA suitable for use in downstream applications such as quantitative RT-PCR. The kit provides the reagents for processing the samples and uses prefilled cartridges for purification, maximizing simplicity and convenience. The Maxwell® 16 Total RNA Purification Kit, Maxwell® 16 Tissue LEV Total RNA Purification Kit and Maxwell® 16 Cell LEV Total RNA Purification Kit are designed for use with the Maxwell® 16 Instrument in either the standard or low elution volume (LEV) configuration. The kits provide high-quality, essentially DNA-free total RNA using novel approaches to selectively remove genomic DNA prior to automated RNA purification. You get enhanced sensitivity and improved confidence in your results for quantitative RT-PCR (qRT-PCR), RT-PCR, cDNA synthesis and other applications. The simple protocols require adding a cleared lysate to the reagent cartridge. Simply place the reagent cartridge into the Maxwell® 16 Instrument, and press start. Purified RNA is obtained in less than 45 minutes of hands-free instrument operation. No post-purification treatment with nuclease, cleanup or concentration is required to achieve superior performance in downstream applications. The Maxwell® 16 Total RNA Purification Kits are General Purpose Medical Devices (GPR) in the USA. Visit: www.promega.com/maxwell16/ for up-todate information. Features: • Enjoy Confidence in Your Application Results: Essentially undetectable contaminating genomic DNA means fewer repeated experiments and unexplained or variable results. • Choose Your Sample Type: Flexibility to purify from tissue, cells, blood and other samples. • Achieve High Yield and High Concentration: High yields and highconcentration total RNA result in better performance in gene expression analysis applications. M1 23 45 67 89 10 11 12 13 14 15 16 Size Cat.# Price (Fr) 48 preps AS1270 350.00 48 preps AS1280 350.00 48 preps AS1050 425.00 48 preps AS1220 425.00 Maxwell® 16 Cell LEV Total RNA Purification Kit 48 preps AS1225 425.00 48 preps AS1155 490.00 M1 23 45 67 89 10 11 12 13 14 15 16 No detectable cross-contamination. Sixteen purification reactions were performed using an input of 25mg of mouse liver lysate (odd lanes) or SV RNA Lysis Buffer alone (even lanes). Panel A. Four-microliter aliquots of each purified sample were resolved by 1.2% agarose gel electrophoresis under denaturing conditions. Lane M, RNA Markers (Cat.# G3191). Panel B. Equivalent volumes (1μl) of each sample were amplified by endpoint RT-PCR using a primer pair specific for a portion of beta actin RNA. A total of five microliters of each amplification reaction was analyzed by 1.2% agarose gel electrophoresis and visualized by ethidium bromide staining. Lane M, 1kb DNA Ladder (Cat.# G5711). simplyRNA TISSUE kidney brain heart lung spleen liver HEK293 HeLa Jurkat CELLS 28S 18S 5S 10001TA 11 Intact RNA Extracted from Tissue Using the Maxwell® 16 LEV simplyRNA Tissue Kit. Extracted tissue samples were run on a FlashGel® System for 5 minutes and signal developed for 15 minutes. The 28S, 18S and 5S are clearly visible indicating intact RNA. For complete and up-to-date product information visit: www.promega.com/catalog 199 Molecular Diagnostics 5967TB Pagina 202
Pagina 204Voor verenigingsbladen, online gidsen en catalogi zie het Online Touch online publisher CMS systeem. Met de mogelijkheid voor een online winkel in uw spaarprogramma.
Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home