Cell Signaling Y Chromosome Deletion Detection System, Version 2.0 Product Y Chromosome Deletion Detection System, Version 2.0 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Y Chromosome Deletion Detection System, Version 2.0, provides a standardized screening panel amplifying only informative nonpolymorphic sequence tag sites (STS) on the human Y chromosome. The system amplifies key functional regions associated with AZoospermia Factor (AZF), namely the regions that flank AZFa and cover AZFb, AZFc, AZFd including DAZ, KAL-Y, SMCY and flanking loci for other key spermatogenesis-related genes (namely RBM1, DFFRY and DBY). Five Multiplex Master Mixes, with a total of 20 characterized Y-specific primer pairs, are included. Four of the multiplex primer sets contain a control primer pair that amplifies a fragment of the X-linked SMCX locus. One of the multiplex primer sets (Multiplex E Master Mix) contains a control primer pair that amplifies a unique region in both male and female DNA (ZFX/ZFY). Finally, a primer pair that amplifies a region of the SRY gene has been included in Multiplex E Master Mix as a control for the testis-determining factor on the short arm of the Y chromosome to detect XX males arising from Y to X translocations. The Multiplex Master Mixes are designed to facilitate the simultaneous amplification of several different regions of the Y chromosome. The amplification products (83–496bp) of the five multiplex PCR amplifications can be clearly separated by agarose gel electrophoresis and visualized by ethidium bromide staining. Failure to amplify specific regions of the Y chromosome is indicative of Y chromosome deletions in the test sample. The size control ladder provided minimizes analysis time and the possibility of misinterpreting molecular weight of amplification products. Features: • Ease of Use: Premixed Multiplex Master Mixes contain 20 primer pairs, including internal controls providing a standardized panel of results requiring no user optimization. • More Robust Reactions: Improved formulation and use of GoTaq® DNA Polymerase minimizes dropouts. • Flexibility: Amplify genomic DNA purified using various methods and with a PE480 (oil overlay) or PE9600/9700 (non-oil overlay) thermal cycler. • Complete System: All required reagents are provided in the kit. Storage Conditions: Store at –20°C. Protocol Y Chromosome Deletion Detection System, Version 2.0 Technical Manual Part# TM248 Size Cat.# Price (Fr) 25 reactions MD1531 2698.00 Primer Sets in the Y Chromosome Deletion Detection System. Locus/ Locus/ Locus/ Locus/ Locus/ Multiplex Master Mix A Master Mix B SMCY/ DYS218/ SYPR3 SY127 SY242 SY152 SY133 SY254 SY157 SY81 SY130 SY182 DAZ/ STS 1 STS 2 STS 3 STS 4 STS 5 DAZ/ DYS240/ DYS271/ DYS221/ Master Mix C DYS219/ DYS212/ DYF51S1/ Master Mix D DYS236/ DYS223/ SY128 SY121 SY145 SY255 DYS215/ DAZ/ Master Mix E SRY/ DYS224/ DYS148/ DYS273/ ZFX1/ SY14 SY134 SY86 SY124 SY84 ZFY 9492LA KAL-Y/ DAZ/ SY208 11 For complete and up-to-date product information visit: www.promega.com/catalog 203 Molecular Diagnostics Pagina 206
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