Science Catalog 2012 Life Cell Signaling Real-Time qPCR and RT-qPCR GoTaq® Real-Time PCR Systems Product GoTaq® qPCR Master Mix GoTaq® 2-Step RT-qPCR System GoTaq® 1-Step RT-qPCR System Available Separately CXR Reference Dye For Research Use Only. Not for Use in Diagnostic Procedures. Description: GoTaq® qPCR Master Mix is a reagent system for use in real-time quantitative PCR (qPCR and RT-qPCR). The system contains BRYT Green® dye, a proprietary fluorescent DNA binding dye that exhibits greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA) than SYBR® Green I. Combined with the GoTaq® Hot Start Polymerase, optimized buffer and proprietary dye, GoTaq® qPCR Master Mix provides robust real-time PCR with increased reliability, reproducibility and sensitivity. GoTaq® qPCR Master Mix is provided as a premixed, ready-to-use stabilized 2X formulation that includes all components for qPCR. GoTaq® 2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) protocol. The components and protocol allow robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibitors, using the GoScript™ Reverse Transcription System and quantification using the GoTaq® qPCR Master Mix. GoTaq® 1-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a one-step reverse transcription-quantitative PCR (RT-qPCR) protocol in a single tube. The BRYT Green® Fluorescent Dye and optimized buffer formulations improve data accuracy and sensitivity of low-expressing targets or targets present at low amounts. Advantages of the GoTaq® Real-Time PCR Systems The BRYT Green® Fluorescent Dye provides brighter dsDNA-dependent fluorescence than SYBR® Green I. With less PCR inhibition than SYBR® Green I, the dye enables efficient amplification, often resulting in earlier quantification cycle (Cq) values and an expanded linear range using the same filters and settings as SYBR® Green I. The CXR reference dye can be detected using the same filters and settings as those used for ROX™. GoTaq® qPCR Master Mix can be used with any real-time instrument capable of detecting SYBR® Green I or FAM™ dye. GoTaq® qPCR Master Mix contains a low level of CXR reference dye. If your real-time thermal cycler requires a high level of reference dye, add CXR Reference Dye to a final concentration of 1X in the reactions. Size Cat.# Price (Fr) 200 reactions A6001 400.00 1,000 reactions A6002 1600.00 50 RT reactions + 200 qPCR reactions A6010 739.00 200 reactions A6020 Pls. Enq. 100 µl C5411 27.00 Features: • Brighter Signal: Sensitive detection for earlier quantitation of low- and high-copy targets. • Enhanced Stability: Exceptional room-temperature setup makes it suitable for automation and high-throughput detection. • Versatile: Compatible with both fast and standard qPCR cycling methods. • Convenient: Read on any instrument capable of reading SYBR® Green I or FAM™ Dye. • Flexible: Compatible with reverse transcriptase (RT) of choice or a complete solution with 1-step and 2-step systems. • Robust: High-efficiency, full-length cDNA synthesis in the presence of inhibitors. • Confidence: The Promega PCR Performance Guarantee. Storage Conditions: Upon arrival, store all components at –20°C, protected from light. For immediate use, components may be stored at 2–8°C, protected from light, for up to 3 months. Protocol GoTaq® qPCR Master Mix Technical Manual GoTaq® 2-Step RT-qPCR System Technical Manual GoTaq® 1-Step RT-qPCR System Technical Manual Part# TM318 TM337 TM355 212 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 215
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