Science Catalog 2012 Life Cell Signaling TNT® Coupled Wheat Germ Extract System Product T® SP6 Coupled Wheat Germ Extract System Size Cat.# Price (Fr) TNT® T3 Coupled Wheat Germ Extract System 40 reactions L4120 947.00 TN 40 reactions L4130 947.00 T® T7/SP6 Coupled Wheat Germ Extract System T® T7/T3 Coupled Wheat Germ Extract System TN 40 reactions L5040 995.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The TNT® Coupled Wheat Germ Extract Systems offer researchers an alternative for eukaryotic cell-free protein expression: a one-tube, coupled transcription/translation system. The TNT® Extract Systems greatly simplify the process and reduce the time required to obtain in vitro translation results. Standard wheat germ extract translations commonly use RNA synthesized in vitro from SP6, T3 or T7 RNA polymerase promoters. This entire process requires separate reactions with several steps between each reaction. The TNT® Extracts bypass many of these steps by incorporating transcription directly in the translation mix. Additionally, the TNT® Extract reactions often produce significantly more protein (two- to sixfold) in a 1.5-hour reaction than do standard in vitro wheat germ extract translations using RNA templates. Magnesium Acetate, 25mM, and Potassium Chloride, 2.5M, can be used to optimize in vitro translation reactions in the TNT® T7 Quick Coupled Transcription/Translation System, Flexi® Rabbit Reticulocyte Lysate System and TNT® Coupled Wheat Germ Extract System. Features: • Reliable: The TNT® Systems are rigorously quality controlled to ensure the highest level of transcription/translation, whether your template is a linear (T3 or T7 only) or circular plasmid. • Convenient: Single-tube procedure eliminates the time and effort required to prepare RNA for a standard wheat germ translation. Translation results can be visualized by autoradiography in 6–8 hours. • Versatile: The T3 system will produce protein from both linear and circular DNA. Use only linear DNA with the T7 system. Use only circular DNA with the SP6 system. For PCR templates use TNT® T7 Quick for PCR DNA (Cat.# L5540). • Controls Included: Luciferase Control DNA and Luciferase Assay Reagents are included with the system as functional controls. Only fulllength luciferase is active. Storage Conditions: Store the polymerase at –20°C. Store the Luciferase Assay Wells at room temperature. Store the other components at –70°C. Avoid multiple freeze-thaw cycles. Protocol TNT® Coupled Wheat Germ Extract Systems Technical Bulletin Part# TB165 TNT® T7 Coupled Wheat Germ Extract System 40 reactions L4140 947.00 TN 40 reactions L5030 995.00 TNT® Starter Bundle Product Size Cat.# Price (Fr) TNT® T7 Quick Starter Bundle, Chemiluminescent 1 each L1210 Pls. Enq. TNT® T7 Quick Starter Bundle, Colorimetric 1 each L1215 Pls. Enq. For Research Use Only. Not for Use in Diagnostic Procedures. Product may not be available in all countries. Please contact you local representative for more information. Description: Get the tools you need in one bundle to start cell-free expression and detection of your proteins of interest. Use the system for in vitro analysis of protein:protein or protein:nucleic acid interactions, or to verify the ability of your clone to express protein. Purchase this special bundle, and get the popular TNT® T7 Quick Coupled Transcription/Translation System, your choice of Transcend™ Translation Detection System, and receive two cell-free expression-qualified expression vectors, pTNT™ and pCMVTNT™ Vectors, at no extra cost. Features: • TNT® T7 Quick Coupled Transcription/Translation System: Our most popular cell-free translation system— a simple one-hour, one-tube reaction. Requires only a protein coding sequence downstream of a T7 RNA polymerase promoter to produce protein. Produced protein may be used in a variety of applications including pull-downs, immunoprecipitations and gel shift assays. TNT® T7 Quick Coupled Transcription/Translation System Technical Manual #TM045. • Transcend™ Translation Detection Systems: A simple addition of the Transcend™ biotin-labeled lysine tRNA to the TNT® T7 Quick reaction provides a simple means of tagging a protein for easy detection. Detect proteins through simple Western blotting techniques with either chemiluminescent or colorimetric techniques. Transcend™ Translation Detection Systems Technical Bulletin #TB182. • pTNT™ Vector: Specifically designed to work with the TNT® Systems with added features to enhance cell-free expression. pTNT™ Vector Technical Bulletin #TB304. • pCMVTNT™ Vector: Specifically designed to work with the TNT® Systems with added features to enhance cell-free expression. Go from cell-free expression to mammalian expression directly with built-in CMV promoter. pCMVTNT™ Vector Technical Bulletin #TB305. Storage Conditions: Store the TNT® Quick System at –70°C. Do not freezethaw the lysate more than two times. Store the Transcend™ tRNA at –70°C. Do not subject the Transcend™ tRNA to more than five freeze-thaw cycles. Store all other Transcend™ System components at 4°C. Store the pTNT™ and pCMVTNT™ Vectors at –20°C. 226 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 229
Pagina 231Heeft u een uitgave, turn pages of e-lesboeken? Gebruik Online Touch: jaarverslag online publiceren.
Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home