Science Catalog 2012 Life Cell Signaling Wheat Germ Extract Product Wheat Germ Extract For Research Use Only. Not for Use in Diagnostic Procedures. Description: Wheat Germ Extract contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors). Wheat Germ Extract is prepared by grinding wheat germ in an extraction buffer followed by centrifugation to remove cell debris. The supernatant is subjected to chromatography that separates endogenous amino acids and plant pigments from the extract. The extract is also treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum. Features: • Optimized: Extract contains an energy-regenerating system (phosphocreatine/phosphocreatine kinase), spermidine (to stimulate the efficiency of chain elongation), magnesium acetate and potassium acetate. • Flexible: Three Amino Acid Mixtures are provided, which enable different choices of radioisotopes. • Robust: Potassium Acetate is provided to enhance translation for a wide range of mRNAs. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –70°C or below. Avoid freeze-thaw cycles. Protocol Wheat Germ Extract Technical Manual T7 Sample System Product T7 Sample System For Research Use Only. Not for Use in Diagnostic Procedures. Description: The T7 Sample System is designed to facilitate the optimization of individual gene expression by offering four unique in vitro translation systems to evaluate. The system consists of samples of: TNT® T7 Quick for PCR DNA, TNT® T7 Quick Coupled Transcription/Translation System, TNT® Coupled Wheat Germ Extract System and E. coli T7 S30 Extract System for Circular DNA. All of the coupled systems utilize RNA generated by a T7 phage promoter. Criteria such as post-translational modifications, ionic optimization and detection methods (i.e., non-isotopic) should be considered when choosing an in vitro system. In some cases only direct experimental results will confirm which system is best for specific genes. Features: • Variety: Four major in vitro translation systems to evaluate. • Value: No requirement for the purchase of several large expensive systems. • Reliability: Comprised of rigorously quality-controlled reagents to ensure the highest level of transcription/translation. • Optimization: Determine which system is best for individual genes. Storage Conditions: Store at –70°C. Protocol T7 Sample System Technical Bulletin Part# TB293 Size Cat.# Price (Fr) 1 each L5900 338.00 Luciferase Control RNA Part# TM230 Product Luciferase Control RNA Size Conc. For Research Use Only. Not for Use in Diagnostic Procedures. Description: Luciferase Control RNA is a unique functional control for in vitro translation reactions. Luciferase Control RNA is an uncapped in vitrotranscribed RNA containing a 30-base poly(A) tail that produces functional luciferase when translated. Control reactions are monitored easily by a luciferase assay for the production of luminescence generated from the full-length luciferase. Features: • Convenient: Control reactions are easily monitored by a luciferase assay for luminescence. • Safe: Non-radioactive format to monitor control activity. Storage Conditions: Store at –70°C. Luciferase SP6/T7 Control DNAs Product Luciferase SP6 Control DNA Luciferase T7 Control DNA For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Luciferase SP6 and T7 Control DNAs are used as functional controls in the TNT® Quick Coupled and TNT® Coupled Transcription/Translation Systems. The Control DNAs contain the gene for luciferase under transcriptional control of a phage RNA polymerase promoter. All constructs carry a 30-base pair poly[d(A)/d(T)] tail following the luciferase gene. Control reactions are monitored easily by the production of luminescence, which is generated from full-length luciferase and the addition of necessary components. Luciferase Control DNAs are supplied as 0.5mg/ml solutions in TE buffer. Storage Conditions: Store at –20°C. Size Cat.# Price (Fr) 20 µg L4741 187.00 20 µg L4821 187.00 Cat.# Price (Fr) 20 µg 1 mg/ml L4561 177.00 Size Cat.# Price (Fr) 5 × 200 µl L4380 286.00 Rabbit Reticulocyte Lysate, Untreated Product Rabbit Reticulocyte Lysate, Untreated For Research Use Only. Not for Use in Diagnostic Procedures. Description: Untreated Rabbit Reticulocyte Lysate contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors) but has not been treated with micrococcal nuclease. Untreated Lysate is used primarily for the isolation of these components and as an abundant source of endogenous globin mRNA. Untreated Lysate is prepared from New Zealand white rabbits in the same manner as treated lysates with the exception that it is not treated with micrococcal nuclease. Features: • Reliable: Consistent reticulocyte production in each lot. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –70°C or below. Protocol Rabbit Reticulocyte Lysate Technical Manual Part# TM232 Size Cat.# Price (Fr) 1 ml L4151 205.00 228 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 231
Pagina 233Heeft u een onderwijscatalogus, nxtbook of internet club bladen? Gebruik Online Touch: cursus naar een digitale publicatie omzetten.
Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home