Cell Signaling Asp-N, Sequencing Grade Product Asp-N, Sequencing Grade For Research Use Only. Not for Use in Diagnostic Procedures. Description: Asp-N, Sequencing Grade, is an endoproteinase that hydrolyzes peptide bonds on the N-terminal side of aspartic and cysteic acid residues: Asp and Cys. Asp-N activity is optimal in the pH range of 4.0–9.0. This sequencing grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in solution or in gel. Storage Conditions: Store at 4°C. Protocol Asp-N Sequencing Grade Product Information Glu-C, Sequencing Grade Product Glu-C, Sequencing Grade For Research Use Only. Not for Use in Diagnostic Procedures. Description: Glu-C, Sequencing Grade (S. aureus V8), is a serine protease that specifically cleaves at the C-terminus of aspartic or glutamic acid residues. In ammonium bicarbonate and ammonium acetate the enzyme specificity is higher at the glutamic residues. In phosphate buffers cleavage occurs at the aspartic and glutamic residues. Glu-C activity is optimal in the pH range of 4.0–9.0. This sequencing grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in solution but not recommended for in-gel digestions. Storage Conditions: Store at 2–10°C. Protocol Glu-C Sequencing Grade Product Information Part# 9PIV165 Size Cat.# Price (Fr) 50 µg V1651 180.00 Part# 9PIV162 Size Cat.# Price (Fr) 2 µg V1621 191.00 Proteinase K Product Proteinase K For Research Use Only. Not for Use in Diagnostic Procedures. Description: Proteinase K, produced by the fungus Tritirachium album Limber, is a serine protease that exhibits broad cleavage activity. It cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids and is useful for general digestion of protein in biological samples. It has been purified to remove RNase and DNase activities. The stability of Proteinase K in urea and SDS and its ability to digest native proteins make it useful for a variety of applications including preparation of chromosomal DNA for pulsedfield gel electrophoresis, protein fingerprinting and removal of nucleases from preparations of DNA and RNA. A typical working concentration for Proteinase K is 50–100μg/ml. Form: Lyophilized powder. Recommended Reaction Buffer: 50mM Tris-HCl (pH 8.0), 10mM CaCl2. Features: • Stable: Active over a pH range of 4.3–12.0, in 0.5% SDS or 1% Triton® X-100 and retains >80% of its activity at temperatures up to 60°C. Storage Conditions: Store lyophilized powder desiccated at –20°C. Protocol Proteinase K Product Information Factor Xa Protease Product Factor Xa Protease For Research Use Only. Not for Use in Diagnostic Procedures. Description: Factor Xa Protease is purified from bovine plasma and activated by treatment with the activating enzyme from Russell’s viper venom. Factor Xa Protease preferentially cleaves after the arginine residue in the amino acid sequence Ile-Glu-Gly-Arg. Recommended Reaction Buffer: 20mM Tris-HCl (pH 7.4), 0.1M NaCl.Storage Conditions: Store in aliquots at –20°C. Size Cat.# Price (Fr) 50 µg V5581 190.00 Part# 9PIV302 Size Cat.# Price (Fr) 100 mg V3021 145.00 13 For complete and up-to-date product information visit: www.promega.com/catalog 235 Protein Expression and Analysis Pagina 238
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