Science Catalog 2012 Life Cell Signaling HaloTag® Mammalian Protein Purification System Product HaloTag® Mammalian Protein Detection and Purification System HaloTag® Mammalian Protein Detection and Purification System Sample Pack Available Separately HaloTEV Protease HaloTag® TMRDirect™ Ligand Protease Inhibitor Cocktail, 50X Size Cat.# Price (Fr) 1 each G6795 980.00 HaloTag® Mammalian Protein Purification System 1 each G6790 800.00 1 each G6799 470.00 1,000 u 5 u/µl G6601 190.00 4,000 u 5 u/µl G6602 570.00 30 µl 0.1 mM G2991 489.00 1 ml G6521 107.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The HaloTag® Mammalian Protein Purification System (Cat.# G6790) is an optimized kit for purification of HaloTag® fusion proteins from mammalian cell culture lysates. HaloTag® fusion proteins form a highly specific and covalent bond with the HaloLink™ Resin. The covalent binding coupled with the low nonspecific binding of the HaloLink™ Resin provides superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels. The HaloTag® Mammalian Protein Detection and Purification System (Cat.# G6795) also includes HaloTag® TMRDirect™ Ligand. The simple-to-use fluorescent detection of the HaloTag® fusion facilitates rapid optimization of expression and purification conditions. Features: • Purify More Protein: HaloLink™ Resin covalently binds >7mg/ml of HaloTag® fusion protein (10X more capacity compared to FLAG®). Recovery is highly efficient, commonly >75%. • Higher Purity: Covalent capture allows extensive and/or stringent washes without loss of bound protein, resulting in very low (<0.1%) nonspecific binding and a highly pure protein. • Easily Scalable: Scale up and down, important for obtaining mg-plus quantities. • Optimized for Mammalian Protein Expression: The HaloTag® platform allows flexibility to move between purification, pull-downs and cellular imaging with a single construct. Storage Conditions: Store the Spin Columns at room temperature. Store the HaloLink™ Resin at 4°C. Store the HaloTEV Protease, Protease Inhibitor Cocktail and HaloTag® TMRDirect™ Ligand at –20°C. Protocol HaloTag Mammalian Protein Detection and Purification Systems Part# TM348 Schematic of the HaloTag® Mammalian Protein Purification System protocol. Streamlined purification process leads to higher purity and recovery of recombinant proteins from cultured cells. 246 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 249
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