Science Catalog 2012 Life Cell Signaling FastBreak™ Cell Lysis Reagent Product FastBreak™ Cell Lysis Reagent, 10X For Research Use Only. Not for Use in Diagnostic Procedures. Description: FastBreak™ Cell Lysis Reagent is designed for the efficient, gentle lysis of E. coli cultures without the need for centrifugation or mechanical cell disruption. The reagent is provided as a 10X concentrate and contains a proprietary nonionic detergent to facilitate lysis. Add the reagent directly to E. coli cultures. Following a brief incubation, the cells are disrupted, and the protein of interest is released. Recombinant proteins can be directly screened in the cell extract or purified by the addition of the appropriate affinity matrix such as the MagneHis™ Protein Purification System. This product is suitable for both manual and automated protocols. Features: • Save Time: Eliminate centrifugation or mechanical disruption. • Easy to Use: Add and incubate. • Flexible: Use manually or on a robotic platform. Storage Conditions: Store at 4–25°C. Protocol FastBreak™ Cell Lysis Reagent Product Information GST Protein Purification MagneGST™ Protein Purification System Product MagneGST™ Protein Purification System Available Separately MagneGST™ Glutathione Particles For Research Use Only. Not for Use in Diagnostic Procedures. Description: The MagneGST™ Protein Purification System provides a simple, rapid and reliable method for the purification of glutathione-S-transferase (GST) fusion proteins. Immobilized glutathione paramagnetic particles (MagneGST™ Particles) are used to isolate GST-tagged protein directly from a crude or cleared lysate using either a manual or automated procedure and requires use of a magnetic stand. GST-tagged proteins can be purified on a small scale from 1ml of culture or on a large scale using more than 50ml of culture. Samples also can be processed using a robotic platform. Features: • Simple: One-step purification of multiple samples with easy handling. • Quick: After cell lysis, no requirement for high-speed centrifugation to clear lysate. • Scalable: Scalable protocol using 1–50ml of cell culture. • Efficient: Achieve high yields with little or no nonspecific background. • Complete: System contains all necessary components except magnetic stand. Storage Conditions: The complete system consists of two individual parts, each with a different storage condition. Store individual boxes at specified temperatures of 4°C and –70°C. Protocol MagneGST™ Protein Purification System Technical Manual Part# TM240 4 ml V8611 240.00 20 ml V8612 1020.00 Size Cat.# Price (Fr) 40 reactions V8600 277.00 200 reactions V8603 1178.00 Part# 9PIV857 Polyhistidine His-Tag Protein Purification Maxwell® 16 Polyhistidine Protein Purification Kit Product Maxwell® 16 Polyhistidine Protein Purification Kit For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Maxwell® 16 Polyhistidine Protein Purification Kit is used with the Maxwell® 16 Instrument to provide an easy method for the efficient, automated purification of polyhistidine-tagged protein from bacterial cultures and other sample types including mammalian and insect cells. With minor modifications, the reagents can also be used for purification of HQ-tagged proteins from bacterial cultures. The Maxwell® Instrument is supplied with a preprogrammed purification procedure and reagent cartridges specifically designed to maximize simplicity and convenience. The instrument can process up to 16 samples in approximately 40 minutes. The purified protein is compatible with downstream applications such as gel electrophoresis and Western blot analysis. Features: • Choose Your Sample Type: Flexibility to purify from multiple starting cultures including bacterial culture, mammalian cells, insect cells and culture medium. • Have Confidence in Your Results: Achieve consistent purification across all samples. • Save Hands-On Time: Prefilled cartridges eliminate reagent preparation, multiple pipetting steps, centrifugation and additional sample manipulation. Storage Conditions: Store at 4°C. Protocol Maxwell® 16 Polyhistidine Protein Purification Kit Technical Manual Part# TM285 Size Cat.# Price (Fr) 48 preps AS1060 425.00 Size Cat.# Price (Fr) 15 ml V8571 55.00 60 ml V8572 193.00 100 ml V8573 290.00 MagneGST™ Pull-Down System Product MagneGST™ Pull-Down System For Research Use Only. Not for Use in Diagnostic Procedures. Description: The MagneGST™ Pull-Down System is designed for detection of protein interactions between GST-fusion proteins expressed in bacterial lysates and prey proteins expressed in the TNT® Systems. Prey protein synthesized in the TNT® Quick Coupled Transcription/Translation Reaction is captured using bait protein (GST-fusion protein) immobilized on MagneGST™ Particles. Nonspecifically bound proteins are then washed away, and the prey protein is analyzed. Prey proteins can be detected by incorporating radioactively labeled methionine in the TNT® Quick reaction, followed by SDS-PAGE and autoradiography or by incorporating the supplied non-radioactive methionine in the TNT® reaction and detecting by Western blotting with protein-specific antibodies. Storage Conditions: Store the TNT® T7 Quick Master Mix and Methionine at –70°C. Store the RQ1 RNase-Free DNase at –20°C. Store the Nuclease-Free Water, MagneGST™ Glutathione Particles, MagneGST™ Binding/Wash Buffer and Cell Lysis Reagent at 4°C. Protocol MagneGST™ Pull-Down System Technical Manual Part# TM249 Size Cat.# Price (Fr) 80 reactions V8870 1056.00 252 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 255
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