Science Catalog 2012 Life Cell Signaling HaloLink™ Protein Array Systems Product HaloLink™ Array (TNT® T7 Quick) Two Slide System HaloLink™ Array (TNT® SP6 Wheat Germ) Two Slide System HaloLink™ Array Six Slide System HaloTag® Standard Protein For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 249. HaloLink™ Magnetic Beads Product HaloLink™ Magnetic Beads For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 250. HaloCHIP™ System Product HaloCHIP™ System For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 251. HaloLink™ Resin Product HaloLink™ Resin Size Cat.# Price (Fr) 1.25 ml G1912 124.00 2.5 ml G1913 225.00 10 ml G1914 540.00 25 ml G1915 945.00 For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 248. HaloTag® Mammalian Pull-Down Systems Product HaloTag® Mammalian Pull-Down and Labeling System HaloTag® Mammalian Pull-Down System HaloTag® Control Vector Available Separately Protease Inhibitor Cocktail, 50X Mammalian Lysis Buffer For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 247. Size Cat.# Price (Fr) 24 reactions G6500 490.00 24 reactions G6504 270.00 20 µg G6591 250.00 1 ml G6521 107.00 40 ml G9381 72.00 Size Cat.# Price (Fr) 20 reactions G9410 783.00 Size Cat.# Price (Fr) 40 reactions G9311 155.00 Size Cat.# Price (Fr) 2 arrays G6140 638.00 2 arrays G6180 638.00 6 slides G6190 1245.00 30 µg G4491 92.00 MagneGST™ Pull-Down System Product MagneGST™ Pull-Down System For Research Use Only. Not for Use in Diagnostic Procedures. For additional information see page 252. Gel Shift Assay Systems Product Gel Shift Assay Core System Gel Shift Assay System Available Separately HeLaScribe® Nuclear Extract, Gel Shift Assay Grade Gel Shift Binding 5X Buffer Size Cat.# Price (Fr) 100 reactions E3050 515.00 100 reactions E3300 645.00 3 × 40 µl E3521 267.00 5 × 200 µl E3581 557.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The gel shift or electrophoretic mobility shift assay provides a simple and rapid method for detecting DNA-binding proteins. This method is widely used to study sequence-specific DNA-binding proteins such as transcription factors. The assay is based on the observation that complexes of protein and DNA migrate through a nondenaturing polyacrylamide gel more slowly than free DNA fragments or double-stranded oligonucleotides. The gel shift assay is performed by incubating a purified protein or a complex mixture of proteins (such as nuclear or cell extract preparations) with a 32P end-labeled DNA fragment containing the putative protein binding site. The reaction products are then analyzed on a nondenaturing polyacrylamide gel. The specificity of the DNA-binding protein for the putative binding site is established by competition experiments using unlabeled DNA fragments or oligonucleotides containing a binding site for the protein of interest or other unrelated DNA sequences. The Core System (Cat.# E3050) includes HeLa Nuclear Extract and SP1 and AP2 Consensus Oligos that can be used as positive controls and serve as a reliable system for obtaining experience with gel shift assays. In addition, the Core System contains T4 Polynucleotide Kinase and Kinase 10X Buffer for labeling oligonucleotides as well as Gel Shift Binding 5X Buffer. Cat.# E3300 contains all of the above plus consensus oligos for AP1, OCT1, CREB, NF-κB, and TFIID. Features: • Positive Controls: The Gel Shift Assay Core System includes a HeLa Nuclear Extract and consensus oligonucleotides for AP2 and SP1. • Versatile: Oligonucleotides can be 5´ end-labeled and used as proteinspecific probes or used as unlabeled oligonucleotides in competition assays. Storage Conditions: Store HeLa Nuclear Extract at –70°C. Store other components at –20°C. Protocol Gel Shift Assay System Technical Bulletin Part# TB110 Size Cat.# Price (Fr) 80 reactions V8870 1056.00 256 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 259
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Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home