Science Catalog 2012 Life Cell Signaling Dual-Reporter Systems Genetic reporters can be used in a wide variety of different applications. Luciferase makes an ideal reporter with great sensitivity, ease of use and quantitation. External Stimuli Receptor Protein-Protein Interactions Viral Infection and Propagation Transduction Signal Nucleus Transcription Factors Promoter Promoter luc Transcription Promoter mRNA Processing Glo Protein Folding Translation Ribozyme siRNA To view an animation about Bioluminescence Assays go to: www.promega.com/multimedia/ Genetic Reporters Selection Guide • Are you performing transient transfection assays? • Are you performing an assay that is designed to result in the decrease of signal, as in those that downregulate cellular responses to stimuli? • Do you require a co-reporter for establishing an internal control or for multiplexing? • Is achieving the highest assay precision (low inter- and intraplate variability) a critical requirement? If YES to Any Dual-Reporter Systems Is limit of detection the critical requirement, even at the expense of throughput and ease of use? YES NO • Is a single reagent addition for higher throughput important to your work? • Is your application volume restricted, such that less reagent volume must be added? NO • Do you have an instrument capable of filtered measurements in luminescence mode? • Do you require genetically similar, dual-color reporters? NO Assay System Luciferase® Reporter DualDual-Glo™ Luciferase Assay System EnduRen™ Live Cell Substrate + Steady-Glo® Luciferase Assay System YES Chroma-Glo™ Luciferase Assay System Bright-Glo™ Luciferase Assay System YES If NO to All Single-Reporter Systems Are sample throughput and ease of use critical requirements? YES Is luminescence intensity a more critical requirement than signal stability? YES NO Which reporter constructs do you wish to use? Firefly Steady-Glo® Luciferase Assay System Renilla EnduRen™ Live Cell Substrate β-gal Beta-Glo® Assay System Which reporter construct do you wish to use? Renilla Luciferase Assay System Renilla Firefly Luciferase Assay System NO Is limit of detection the critical requirement, even at the expense of throughput and ease of use? NO YES luc Deletion Analysis Glo luc Glo Cytoplasm This chart and the chart on page 141 provide a general guide for selecting from Promega luminescence reporter assays and firefly and Renilla luciferase genes. These charts contain general questions about your application needs and preferences and are designed to recommend a product based on your answer(s). Selection of a particular reporter assay or gene may require additional considerations and preferences not addressed by these charts. In these instances, please contact Technical Services for assistance. 260 For complete and up-to-date product information visit: www.promega.com/catalog 5678MH 4311MA09_3B Pagina 263
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