Cell Signaling Luminogenic Enzyme Substrates Product Luciferin Detection Reagent Luciferin Detection Reagent with esterase Luciferin-NAT2 Luciferin-3A7 Luciferin-4A Luciferin-4F2/3 Luciferin-4F12 Luciferin-2J2/4F12 (ester) Luciferin-MultiCYP (ester) For Research Use Only. Not for Use in Diagnostic Procedures. Description: The proluciferin substrates can be used to monitor the activity of specific isoforms of cytochrome P450 or NAT2 as indicated in the name of the substrate. The Luciferin-MultiCYP is a promiscuous substrate that reacts with at least 21 P450 isoforms and is useful for measuring net CYP activity in a mixed population of P450s. The Luciferin-NAT2 is an excellent substrate for Nacetyltransferase 2 (NAT2), a phase II biotransformation enzyme that acetylates aromatic amine groups on xenobiotic compounds. This substrate shows little to no cross-reactivity with NAT1. Pgp-Glo™ Assay Systems Product Pgp-Glo™ Assay System Pgp-Glo™ Assay System with P-glycoprotein For Research Use Only. Not for Use in Diagnostic Procedures. Size Cat.# Price (Fr) 10 ml V3591 411.00 10 ml V3601 826.00 Description: The Pgp-Glo™ Assay Systems provide the necessary reagents for performing luminescent P-glycoprotein (Pgp) ATPase assays. Pgp, also known as MDR1 and ABCB1, is a 170kDa integral plasma membrane protein that functions as an ATP-dependent drug efflux pump and plays an important role in multidrug resistance and certain adverse drug-drug interactions. Compounds that interact with Pgp can be identified as stimulators or inhibitors of its ATPase activity. Compounds that are substrates for transport by Pgp typically stimulate its ATPase activity. The Pgp-Glo™ Assay detects the effects of compounds on recombinant human Pgp in a cell membrane fraction. The assay relies on the ATP dependence of the light-generating reaction of firefly luciferase. ATP is first incubated with Pgp; then the Pgp ATPase reaction is stopped, and the remaining unmetabolized ATP is detected as a luciferase-generated luminescent signal. Pgp-dependent decreases in luminescence reflect ATP consumption by Pgp; thus the greater the decrease in signal, the higher the Pgp activity. Accordingly, samples containing compounds that stimulate the Pgp ATPase will have significantly lower signals than untreated samples. Storage Conditions: Store Recombinant Human Pgp Membranes at –70°C. All other components can be stored at –70°C or –20°C, protected from light. Protocol Pgp-Glo™ Assay Systems Technical Bulletin Part# TB341 Size Cat.# Price (Fr) 50 ml V8921 580.00 10 ml V8920 145.00 50 ml V8931 580.00 10 ml V8930 145.00 3 mg P1721 309.00 3 mg P1741 309.00 3 mg P1621 309.00 3 mg P1651 309.00 3 mg P1661 309.00 3 mg P1671 309.00 3 mg P1731 309.00 Features: • Complete System: Cat.# V3591 includes all the reagents required to run the assay except the P-glycoprotein: Pgp reaction buffer, MgATP, Verapamil, Na3VO4, and a lyophilized ATP detection reagent and its reconstitution buffer. Cat.# V3601 includes all the reagents provided in the Pgp-Glo™ System with the addition of Recombinant Human Pgp Membranes to provide a completely optimized kit. • Stable Activities: “Glow-type” signal allows processing of multiple samples without concern of variability over time. • Low False-Positive Rate: Use of a proprietary stabilized firefly luciferase and a proprietary luciferase assay formulation minimizes the incidence of false positives due to inhibition of luciferase by analytes when screening for compounds that affect Pgp activity. • Simple: The simple protocol makes the assay amenable to high-throughput screening in multiwell plates. 2 For complete and up-to-date product information visit: www.promega.com/catalog 23 Cell Health Assays Pagina 26
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