Cell Signaling Apoptosis       VIABLE T0                            NECROSIS               APOPTOSIS T2                                REDUCED VIABILITY T1 2  Assay Type CellTiter-Glo® Assay CellTiter-Fluor™ Assay CellTiter-Blue® Assay CellTiter 96® AQueous One Solution Assay MultiTox-Glo Assay MultiTox-Fluor Assay CytoTox-Glo™ Assay CytoTox-Fluor™ Assay CytoTox-ONE™ Assay Caspase-Glo® 3/7 Assay Parameter/Biomarker Measured Viable cell ATP Live-cell protease Resazurin reduction by NADH MTS reduction by NADH Viability and cytotoxicity by live- and dead-cell proteases Viability and cytotoxicity by live- and dead-cell proteases Dead-cell protease release Dead-cell protease release LDH release Caspase-3/7 activity Apo-ONE® Caspase 3/7 Assay Caspase-3/7 activity Caspase-Glo® 8 Assay Caspase-Glo® 9 Assay GSH-Glo™ Assay GSH/GSSG-Glo™ Assay Caspase-8 activity Caspase-9 activity Mitochondrial ToxGlo™ Assay ATP Dead cell protease GSH GSH/GSSG Time to Results Sensitivity (*384 well) Plate Format Instrument 10 minutes 10 viable cells* 96/384/1536 Luminometer/CCD 0.5–3 hours 40 viable cells 96/384/1536 1–4 hours 50 cells* 1–4 hours 200 cells* 0.5 hour 40 viable cells, 10 dead cells 0.5–3 hours 40 live cells, 10 dead cells 15 minutes 10 dead cells 0.5–3 hours 10 dead cells 10 minutes 200 cells* 0.5 hour 20 cells* 1–18 hours 200 cells* 0.5 hour 0.5 hour 1 hour 30 minutes 1hour ~1000 cells ~1500 cells 96/384/1536 96/384 96/384/1536 96/384/1536 Fluorometer AFC 400nmEx/505nmEm Fluorometer, Resorufi n 560nmEx/590nmEm Spectrophotometer Abs 490nm AFC 400nmEx/505nmEm Luminometer Fluorometer Fluorometer AFC 400nmEx/505nmEm R110 485nmEx/520nmEm 96/384/1536 Luminometer 96/384 96/384 Fluorometer R110 485nmEx/520nmEm Fluorometer, Resorufi n 560nmEx/590nmEm 96/384/1536 Luminometer 96/384/1536 96 96 10 viable cells 96/394 10 dead cells 96\384 96\384 Fluorometer R110 499nmEx/521nmEm Luminometer Luminometer Luminometer Luminometer Luminometer 9481LA For complete and up-to-date product information visit: www.promega.com/catalog 25 Cell Health Assays  Pagina 28

Pagina 30

Interactieve ekrant, deze PDF of onderwijscatalogus is levensecht online geplaatst met Online Touch en bied het van pdf naar online converteren van digitale onderwijs catalogi.

Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home