Science Catalog 2012 Life Cell Signaling PowerPlex® 16 HS System Product PowerPlex® 16 HS System Size Cat.# Price (Fr) 100 reactions DC2101 3253.00 400 reactions DC2100 11386.00 DG1071, DW0991 For Laboratory Use. DC2101, DC2100, DD1001 Not For Medical Diagnostic Use. For additional information see page 154. Stemness Assessment StemElite™ Gene Expression System Product StemElite™ Gene Expression System StemElite™ Gene Expression System Plus Size Cat.# Price (Fr) 100 qPCR reactions B1001 284.00 100 qPCR reactions + 50 RT reactions B1002 559.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The StemElite™ Gene Expression System is a novel real-time quantitative PCR (qPCR) system for detection and relative quantification of RNA expression levels associated with the differentiation state or ‘potency’ of cells. The StemElite™ Gene Expression System is optimized to quantitatively amplify a two-color duplex, enabling the user to amplify a transcript of interest as well as a reference gene in a single reaction. Features: • Achieve sensitive real-time detection and relative quantification of RNA expression levels for monitoring stem cell differentiation in a multiplex amplification. • Quantitatively amplify a two-color duplex. • Amplify in a single tube the transcript of interest and reference transcript (GAPDH or Actb). • Reduce the number of reactions required to measure multiple transcripts. • Improve experimental data quality by measuring all transcripts in the same well. Storage Conditions: Store at –20°C. Protocol StemElite™ Gene Expression System Technical Manual Part# TM323 StemElite™ Human Pluripotent Transcripts Product StemElite™ NANOG/GAPDH Primer Pair (20X) StemElite™ SOX2/GAPDH Primer Pair (20X) StemElite™ POU5F1/GAPDH Primer Pair (20X) StemElite™ LIN28/GAPDH Primer Pair (20X) StemElite™ KLF4/GAPDH Primer Pair (20X) StemElite™ MYC/GAPDH Primer Pair (20X) For Research Use Only. Not for Use in Diagnostic Procedures. Description: NANOG, SOX2, POU5F1, LIN28, KLF4 and MYC are functionally associated with maintenance of the undifferentiated human embryonic stem cell. Features: • Achieve sensitive real-time detection and relative quantification of RNA expression levels for monitoring stem cell differentiation in a multiplex amplification. • Quantitatively amplify a two-color duplex. • Amplify in a single tube the transcript of interest and reference transcript (GAPDH). • Reduce the number of reactions required to measure multiple transcripts. • Improve experimental data quality by measuring all transcripts in the same well. Storage Conditions: Store at –20°C. scripts Product StemElite™ Human Heart-Associated TranStemElite™ NPPA/GAPDH Primer Pair (20X) StemElite™ MYL7/GAPDH Primer Pair (20X) StemElite™ MYL2/GAPDH Primer Pair (20X) StemElite™ MYH6/GAPDH Primer Pair (20X) StemElite™ MYH7/GAPDH Primer Pair (20X) StemElite™ NKX2-5/GAPDH Primer Pair (20X) StemElite™ TNNT2/GAPDH Primer Pair (20X) StemElite™ TNNI3/GAPDH Primer Pair (20X) StemElite™ MEF2C/GAPDH Primer Pair (20X) StemElite™ PLN/GAPDH Primer Pair (20X) StemElite™ GATA4/GAPDH Primer Pair (20X) For Research Use Only. Not for Use in Diagnostic Procedures. Description: Pluripotential stem cells can give rise to differentiated cells and tissues for all three embryonic germ layers. NPPA, MYL7, MYL2, MYH6, MYH7, NKX2-5, TNNT2, TNNI3, MEF2C, PLN and GATA4 are mesodermal markers associated with differentiation of cardiac muscle. Features: • Achieve sensitive real-time detection and relative quantification of RNA expression levels for monitoring stem cell differentiation in a multiplex amplification. • Quantitatively amplify a two-color duplex. • Amplify in a single tube the transcript of interest and reference transcript (GAPDH). • Reduce the number of reactions required to measure multiple transcripts. • Improve experimental data quality by measuring all transcripts in the same well. Storage Conditions: Store at –20°C. Size Cat.# Price (Fr) 100 µl B1071 133.00 100 µl B1081 133.00 100 µl B1091 133.00 100 µl B1101 133.00 100 µl B1111 133.00 100 µl B1121 133.00 100 µl B1131 133.00 100 µl B1141 133.00 100 µl B1151 133.00 100 µl B1161 133.00 100 µl B1171 133.00 Size Cat.# Price (Fr) 100 µl B1011 133.00 100 µl B1021 133.00 100 µl B1031 133.00 100 µl B1041 133.00 100 µl B1051 133.00 100 µl B1061 133.00 296 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 299
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