Cell Signaling GSH-Glo™ Glutathione Assay Product GSH-Glo™ Glutathione Assay For Research Use Only. Not for Use in Diagnostic Procedures. Description: The GSH-Glo™ Assay is a luminescent-based assay for the detection and quantification of glutathione (GSH) in cells or biological samples. A change in GSH levels is important for assessing toxicological responses and is an indicator of oxidative stress, potentially leading to apoptosis or cell death. The assay is based on the conversion of a luciferin derivative into luciferin in the presence of GSH. The reaction is catalyzed by a glutathione S-transferase (GST) enzyme supplied in the kit. The luciferin formed is detected in a coupled reaction using Ultra-Glo™ Recombinant Luciferase that generates a glow type luminescence that is proportional to the amount of glutathione present in cells. The assay provides a simple, fast and sensitive alternative to colorimetric and fluorescent methods and can be adapted easily to high-throughput applications. Features: • Fast: Results in as little as 30 minutes. • Simplified Method: The simple two-reagent-addition assay minimizes the number of assay steps compared to conventional GSH assays and is adapted easily to higher throughput applications. No deproteination step required! • Greater Sensitivity: The luminescent method avoids inherent background fluorescence associated with other methods thereby providing excellent signal-to-background ratios. • Stable Signal: Half-life greater than 5 hours. Storage Conditions: Store at –20°C protected from light. Protocol GSH-Glo™ Glutathione Assay Technical Bulletin Part# TB369 Luminometer Schematic showing GSH-Glo™ Assay procedure. Size Cat.# Price (Fr) 10 ml V6911 604.00 50 ml V6912 2114.00 Luc-NT GST Enzyme Reaction Buffer GST-Glo™ Add GSH-Glo™ Reagent to plate. Incubate 30 minutes. Luciferin Detection Buffer Remove medium. 2 reconstituted Luciferin Detection Reagent Add reconstituted Luciferin Detection Reagent to plate. Incubate 15 minutes. Place plate in luminometer. Read. For complete and up-to-date product information visit: www.promega.com/catalog 45 Cell Health Assays 6957MB Pagina 48
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