Science Catalog 2012 Life Cell Signaling Protein Kinase Assays ADP-Glo™ Kinase Assay Product ADP-Glo™ Kinase Assay Size Cat.# Price (Fr) 1,000 assays V9101 891.00 10,000 assays V9102 4455.00 100,000 assays V9103 Pls. Enq. For Research Use Only. Not for Use in Diagnostic Procedures. Description: ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase. The luminescent signal positively correlates with kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. The assay is performed in two steps; first, after the kinase reaction, an equal volume of ADP-Glo™ Reagent is added to terminate the kinase reaction and deplete the remaining ATP. In the second step, the Kinase Detection Reagent is added, which simultaneously converts ADP to ATP and allows the newly synthesized ATP to be measured using a coupled luciferase/luciferin reaction. The ADP-Glo™ Kinase Assay has a high dynamic range and produces a strong signal at low ATP to ADP conversion, making it well suited for screening low activity kinases such as growth factor receptor tyrosine kinases. The assay produces minimal false hits and Z´ values of greater than 0.8. Several Kinase Enzyme Systems are available. Visit www.promega.com/kinase/ to see the collection. Features: • High Signal Strength at Low ATP Conversion: Users can measure kinase activity that more closely mimics physiological conditions. • Sensitive: The assay is sensitive to low concentrations of ADP, thus requiring less enzyme than other assays. • Universal: The assay can be used with virtually with any kinase—enables researchers to screen a wider range of kinases in-house, reducing dependency on costly outsourcing of kinase selectivity profiling. • Accurate: Accurately measures ADP levels at a wide range of starting ATP concentrations; users assured that activity measured truly reflects kinase activity and produces accurate IC50s comparable to radioactivity-based assays. • Accommodate Wide Range of ATP Levels: The assay can be used at ATP concentrations up to 1mM, important for kinases with high Km values for ATP. • Stable Luminescent Signal: Users can perform batch plate processing without need for strictly timed incubations; flexible. Storage Conditions: Store the system at –20°C. Before use, thaw all reagents completely at room temperature. Once thawed, components should be thoroughly mixed before use. Once prepared, the Kinase Detection Reagent (Kinase Detection Buffer + Substrate) should be divided into aliquots and stored at –20°C. Protocol ADP-Glo™ Kinase Assay Technical Manual Part# TM313 54 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 57
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