Science Catalog 2012 Life Cell Signaling Biochemical Buffers and Reagents 4-CORE® Buffer Pack Product 4-CORE® Buffer Pack (Buffers A, B, C and D), 1ml each For Laboratory Use. For additional information see page 88. 5M Sodium Chloride, Molecular Biology Grade Product 5M Sodium Chloride, Molecular Biology Grade Size Conc. Cat.# Price (Fr) 1L 5M V4221 53.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: 5M Sodium Chloride is commonly used in many molecular biology and forensic applications. Form: Clear, colorless liquid. Composition: 292.2g/L NaCl in deionized water. Properties: • pH at 25°C (1M): 5.0–8.0. • A260 at 5M: ≤0.02. • A280 at 5M: ≤0.01. • Conductivity at 25°C (0.05M): 5,000–7,000μSm. Features: • Quality Tested: Each lot of NaCl is tested and certified to be free of DNase, RNase and protease activity. Storage Conditions: Store at 22–25°C. Acrylamide, Molecular Grade Product Acrylamide, Molecular Grade For Research Use Only. Not for Use in Diagnostic Procedures. Description: Acrylamide, Molecular Grade, is used for the electrophoretic separation of nucleic acids and proteins. Very small DNA fragments, such as those generated by sequencing reactions, can be resolved by polyacrylamide gel electrophoresis. Proteins can be separated by a variety of techniques, including denaturing gel electrophoresis using SDS or urea, isoelectric focusing and native gel electrophoresis in a wide variety of buffers. Formula Weight: 71.08. Form: White, free-flowing crystals. Properties: • Purity: ≥99.9%. • Melting Point: 84–86°C. • Free Acrylic Acid: <0.001%. • Iron: ≤1ppm. • Lead: ≤1ppm. • pH (10% in 0.1M NaCl at 25°C): 6.0–7.0. • Conductivity (40% in water): ≤2.5μmhos. Features: • Quality Tested: Each lot of Molecular Grade Acrylamide is tested and certified to be free of DNase, RNase and protease activity. Storage Conditions: Store at 22–25°C. Protect from moisture. Size Cat.# Price (Fr) 100 g V3111 74.00 500 g V3115 259.00 Size Cat.# Price (Fr) 4ml R9921 43.00 AgarACE™ Enzyme Product AgarACE™ Enzyme For Laboratory Use. For additional information see page 95. Agarose, LE, Analytical Grade Product Agarose, LE, Analytical Grade For Research Use Only. Not for Use in Diagnostic Procedures. Description: Agarose, LE, Analytical Grade, is used for the electrophoretic separation of nucleic acids. Form: White powder. Properties: • Gel Strength (1%): ≥1,000g/cm2. • Gelling Point (1.5%): 36–39°C. • Melting Point (1.5%): 87–89°C. • EEO (–mr): 0.09–0.13. • Sulfate: ≤0.14%. • Moisture: ≤7.0%. Features: • Quality Tested: Each lot of Analytical Grade LE Agarose is tested and certified to be free of DNase and RNase activity. Storage Conditions: Store at 22–25°C. Agarose, LMP, Preparative Grade for Large Fragments (>1,000bp) Product Agarose, LMP, Preparative Grade for Large Fragments (>1,000bp) For Research Use Only. Not for Use in Diagnostic Procedures. Description: Agarose, LMP, Preparative Grade for Large Fragments (>1,000bp), is a premium agarose used for isolating DNA fragments larger than 1,000bp. Each lot is tested and certified for the following applications: 1) restriction digestion, 2) ligation and transformation, and 3) random prime labeling. LMP = low melting point (i.e., ≤65°C). Form: White powder. Properties: • Gelling Point (1.5%): 26–30°C. • Melting Point (1.5%): ≤65°C. • Sulfate: ≤0.10%. • EEO (–mr): ≤0.10. • Moisture: ≤10%. • Gel Strength (1%): ≥200g/cm2. Features: • Quality Tested: Each lot of Preparative Grade LMP Agarose is tested and certified to be free of DNase and RNase activity. Storage Conditions: Store at 22–25°C. Size Cat.# Price (Fr) 25g V2831 335.00 Size Cat.# Price (Fr) 100g V3121 215.00 500g V3125 753.00 Size Conc. Cat.# Price (Fr) 25u 0.15–0.30u/µl M1741 132.00 500u 0.15–0.30u/µl M1743 1320.00 2 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 5
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