Cell Signaling Casein Kinase I Product Casein Kinase I For Research Use Only. Not for Use in Diagnostic Procedures. Description: Casein Kinase I (CKI or CK-1) is a ubiquitous and highly conserved serine/threonine protein kinase found in eukaryotic cells. CKI exists in multiple forms in mammalian tissue and is present in the nucleus, cytosol, plasma membrane and microsomes. CKI isolated from most species is a 35–37kDa monomer. In contrast to Casein Kinase II, CKI primarily uses Mg2+/ ATP as the phosphate donor and is not sensitive to heparin inhibition. Storage Conditions: Store at –20°C. Protocol Casein Kinase I Product Information Casein Kinase II Product Casein Kinase II For Research Use Only. Not for Use in Diagnostic Procedures. Description: Casein Kinase II (CKII or CK-2) is a ubiquitous serine/threonine protein kinase found in eukaryotic cells and is also known as phosvitin kinase, glycogen synthase 5 kinase, troponin T kinase and casein kinase G. CKII is a multifunctional protein kinase implicated in a variety of cellular processes and functions, including mitosis and cellular transformation. CKII isolated from most species is composed of α and α´ subunits (37–44kDa) and β subunits (24–28kDa). The holoenzyme exists as an αα´β2 tetramer. The α subunit contains the catalytic domain, whereas the β subunits presumably regulate the catalytic activity of the holoenzyme. Casein Kinase II is purified from rat liver. Storage Conditions: Store at –70°C. Protocol Casein Kinase II Product Information Part# 9PIV562 Size Cat.# Price (Fr) 100 u V5621 297.00 Part# 9PIV563 Size Cat.# Price (Fr) 100 u V5631 297.00 EGF Receptor Product EGF Receptor For Research Use Only. Not for Use in Diagnostic Procedures. Description: Epidermal Growth Factor Receptor (EGF Receptor) is a cellsurface glycoprotein composed of a single polypeptide chain that binds the peptide Epidermal Growth Factor (EGF). The EGF Receptor consists of an extracellular ligand binding domain, a single transmembrane region and a cytoplasmic intrinsic tyrosine kinase domain. Upon ligand binding, the EGF Receptor autophosphorylates, activating the tyrosine kinase domain of the EGF Receptor. EGF Receptor is immunopurified from the A431 cell line following a procedure detailed by Weber et al.. The purified EGF Receptor does possess tyrosine kinase activity due to the bound EGF; however, the EGF Receptor has not been autophosphorylated. Storage Conditions: Store at –70°C. Protocol EGF Receptor Protocol Protein Kinase C Product Protein Kinase C Size Conc. For Research Use Only. Not for Use in Diagnostic Procedures. Description: Protein Kinase C is an 82kDa monomeric enzyme consisting of a C-terminal catalytic domain and a cysteine-rich N-terminal regulatory domain. The regulatory domain contains the sites for calcium and phospholipid binding and a pseudosubstrate subdomain, the target for PKC autophosphorylation. PKC is isolated from rat brain following the procedure of Walton et al. The purified PKC consists primarily of α, β and γ isoforms with lesser amounts of Δ and ζ isoforms. Features: • Highly Pure: PKC is greater than 90% pure as determined by SDS-PAGE. Storage Conditions: Store at –70°C. Protocol Protein Kinase C Product Information Part# 9PIV526 Cat.# Price (Fr) 1 µg 25 µg/ml V5261 554.00 Part# 9PIV555 Size Cat.# Price (Fr) 10 u V5551 762.00 3 For complete and up-to-date product information visit: www.promega.com/catalog 65 Cell Signaling Pagina 68
Pagina 70Interactieve digi krant, deze verenigingsblad of onderwijscatalogus is levensecht online geplaatst met Online Touch en bied het online bladerbaar uitgeven van digitale tijdschriften.
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