Cell Signaling Product Anti-ACTIVE® JNK pAb, Rabbit, (pTPpY) Size Cat.# Price (Fr) 40 µl V7931 804.00 120 µl V7932 1327.00 Product Anti-ACTIVE® MAPK pAb, Rabbit, (pTEpY) Anti-ACTIVE® p38 pAb, Rabbit, (pTGpY) A. Anti-ACTIVE® MAPK kDa – + NGF 98 – 64 – 50 – Immunocytochemical detection of active JNK enzyme in Drosophila pupal retina using the Anti-ACTIVE® JNK pAb. Drosophila pupal retina at 25% of pupal development were fixed in 3% paraformaldehyde in PBS. The Anti-ACTIVE® JNK pAb was diluted 1:100 in PBS containing 10% fetal bovine serum and 0.2% Triton® X-100. Samples were incubated with the primary antibody overnight at 4˚C, washed 3 times (10 minutes each) with 0.2% Triton® X-100 and then incubated with a goat anti-rabbit Cy®3 conjugate for 2 hours at 4˚C. The results illustrate the presence of dually phosphorylated, active forms of JNK in discrete structures of the fly retinal ommatidia including intense staining of the inner cone cells as well as the mechanosensory bristles and surrounding pigment cells. The pattern of staining (which was distinct from results obtained with an antibody for active p38) and the absence of staining in control experiments (data not shown) support the high specificity of the AntiACTIVE® JNK pAb. Image kindly provided by David T. Miller and Ross Cagan, Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri. 36 – 1 2 B. Anti-MAPK kDa – + NGF 98 – 64 – 50 – 36 – 1 2 3 4 5 6 Detection of MAPK, JNK and p38 in PC12 cell extracts. Panel A. Western blot analysis using Anti-ACTIVE® MAPK, Anti-ACTIVE® JNK and Anti-ACTIVE® p38 polyclonal antibodies to detect activated MAPK, JNK and p38. Panel B. Western blot analysis using anti-MAPK, anti-JNK and anti-p38 antibodies to detect activated and nonactivated MAPK, JNK and p38 in untreated or NGF- or sorbitol-treated PC12 cells. 3 45 6 Anti-JNK – + Sorbitol Anti-p38 – + Sorbitol Anti-ACTIVE® JNK – + Sorbitol Size Cat.# Price (Fr) 40 µl V8031 623.00 100 µl V1211 590.00 Anti-ACTIVE® p38 – + Sorbitol 3 – ERK1 – ERK2 – JNK2 – JNK1 – p38 – ERK1 – ERK2 – JNK2 – JNK1 – p38 For complete and up-to-date product information visit: www.promega.com/catalog 67 Cell Signaling 1825TA07_7A 1859TC Pagina 70
Pagina 72Interactieve digi spaarprogramma, deze vaktijdschrift of pdf is levensecht online geplaatst met Online Touch en bied het digitaal zetten van e-relatiemagazines.
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