Science Catalog 2012 Life Cell Signaling Molecular Weight Markers BenchTop DNA Markers Product BenchTop ΦX174 DNA/HaeIII Markers BenchTop pGEM® DNA Markers BenchTop PCR Markers BenchTop 1kb DNA Ladder BenchTop 100bp DNA Ladder For Laboratory Use. Size Cat.# Price (Fr) 250 µl G7511 152.00 250 µl G7521 152.00 300 µl G7531 152.00 600 µl G7541 131.00 300 µl G8291 114.00 Description: The BenchTop DNA Markers offer the convenience of storage at room temperature (22–25°C) as well as the capability of direct loading onto agarose gels. The BenchTop DNA Markers are supplied in a stabilizing solution of 1X Blue/Orange Loading Dye, which circumvents any requirements for further manipulation. BenchTop ΦX174 DNA/HaeIII Markers: Eleven phenol-extracted, ethanolprecipitated DNA fragments ranging in size from 72bp to 1,353bp. BenchTop pGEM® DNA Markers: Fifteen phenol-extracted, ethanol-precipitated DNA fragments ranging in size from 36bp to 2,645bp. These unique markers are generated from separate digests of pGEM®-3 Vector DNA with HinfI, RsaI and SinI later combined to form the markers. BenchTop PCR Markers: Six bands of equal intensity of 50, 150, 300, 500, 750, and 1,000bp. The BenchTop PCR Markers may be run on polyacrylamide gels with less loading volume; however, additional bands may be visible compared to those visible on agarose gels. bp – 1,353 bp – 1,078 – 872 – 603 – 310 – 118 – 72 – 2,645 bp – 1,605 – 1,198 – 676 – 281/271 – 234 – 194 – 517 – 460 – 396 – 350 – 222 – 179 – 126 – 75/65 [51,36] bp – 1,000 – 750 – 500 – 300 – 150 – 50 – 4,000 – 5,000 – 6,000 – 8,000 – 10,000 – 2,000 – 2,500 – 3,000 – 1,500 – 500 – 750 – 1,000 – 250, 253 2% agarose BenchTop φX174 DNA/HaeIII Markers Cat.# G7511 Load 5µl/lane. 2% agarose BenchTop pGEM® DNA Markers Cat.# G7521 Load 5µl/lane. 2% agarose BenchTop PCR Markers Cat.# G7531 Load 6µl/lane. 0.7% agarose BenchTop 1kb DNA Ladder Cat.# G7541 Load 6µl/lane. 2% agarose BenchTop bp – 1,500 – 1,000 – 900 – 800 – 700 – 600 – 500 – 400 – 300 – 200 – 100 BenchTop 1kb DNA Ladder: Thirteen blunt-ended fragments with sizes ranging from 250bp to 10,000bp. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromide-stained agarose gels for easy identification. All other fragments are of equal intensity. The ladder is dephosphorylated and ready for 5´ end-labeling with radioisotopes using T4 Polynucleotide Kinase, allowing visualization by autoradiography. BenchTop 100bp DNA Ladder: Eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an additional band at 1,500bp. The 500bp fragment is present at increased intensity for easy identification. The ladder is dephosphorylated and ready for 5´ end-labeling with radioisotopes using T4 Polynucleotide Kinase, allowing visualization by autoradiography. Recommended Loading: Cat.# G7511, G7521: Load 5μl/lane. Cat.# G7531, G7541, G8291: Load 6μl/lane. Features: • Convenient: Storage at 22–25°C. • Efficient: Premixed with loading buffer. Ready to load onto agarose gels. • Versatile: Five different BenchTop DNA Markers available. Storage Conditions: Store at 22–25°C. 100bp DNA Ladder Cat.# G8291 Load 6µl/lane. 72 For complete and up-to-date product information visit: www.promega.com/catalog 8651TA Pagina 75
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