Science Catalog 2012 Life Cell Signaling DNA Ladders Product PCR Markers 100bp DNA Ladder 1kb DNA Ladder For Laboratory Use. Size Conc. Cat.# Price (Fr) 250 µl ~0.06 µg/µl G3161 170.00 250 µl 0.13 µg/µl G2101 134.00 500 µl 0.1 µg/µl G5711 209.00 Description: The DNA Ladders are ladders with defined sizes. The ladders are not intended for use in quantitative analysis. Each ladder is provided with a tube of 6X Blue/Orange Loading Dye. PCR Markers: Six bands of equal intensity of 50, 150, 300, 500, 750 and 1,000bp. The PCR Markers may be run on polyacrylamide gels with less loading volume; however, additional bands may be visible compared to those visible on agarose gels. bp – 1,500 – 1,000 – 900 – 800 – 700 bp – 1,000 – 750 – 500 – 300 – 150 – 50 – 600 – 500 – 400 – 300 – 200 – 100 – 4,000 – 5,000 – 6,000 – 8,000 – 10,000 – 2,000 – 2,500 – 3,000 – 1,500 – 500 – 750 – 1,000 – 250, 253 2% agarose PCR Markers Cat.# G3161 Load 5µl/lane. 2% agarose 100bp DNA Ladder Cat.# G2101 Load 5µl/lane. 0.7% agarose 1kb DNA Ladder Cat.# G5711 Load 5µl/lane. 100bp DNA Ladder: Eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an additional band at 1,500bp. The 500bp fragment is present at increased intensity for easy identification. The ladder is dephosphorylated and ready for 5´ end-labeling with radioisotopes using T4 Polynucleotide Kinase, allowing visualization by autoradiography. 1kb DNA Ladder: Thirteen blunt-ended fragments with sizes ranging from 250bp to 10,000bp. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromide-stained agarose gels for easy identification. All other fragments are of equal intensity. The ladder is dephosphorylated and ready for 5´ end-labeling with radioisotopes using T4 Polynucleotide Kinase, allowing visualization by autoradiography. Recommended Loading: Load 5μl/lane. Storage Conditions: Store at –20°C. bp 74 For complete and up-to-date product information visit: www.promega.com/catalog 0094TA05_3A 0973TC03_5A 1409TA03_6A 8654TA Pagina 77
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