Science Catalog 2012 Life Cell Signaling XmaI Product XmaI For Laboratory Use. Description: C▼CCGG G G GGCC▲C Storage Conditions: Store at –20°C. XmnI Product XmnI For Laboratory Use. Description: GAANN▼NNT TC CT TNN▲NNAAG Storage Conditions: Store at –20°C. MULTI-CORE™ Buffer Pack Product MULTI-CORE™ Buffer Pack For Laboratory Use. Size Cat.# Price (Fr) 3 × 1 ml R9991 43.00 Description: The MULTI-CORE™ Buffer Pack contains convenient aliquots of the Promega universal restriction enzyme 10X buffer. The MULTI-CORE™ Buffer is formulated to provide simple buffering conditions for performing multiple digestions. Many Promega restriction enzymes have between 50% and 100% activity in reactions using MULTI-CORE™ Buffer. Features: • Convenient and Economical: MULTI-CORE™ Buffer enables codigestion of DNA with more than one enzyme in a single reaction. In most cases, only modest adjustments in the amount of enzyme used will ensure complete multiple digestions. Storage Conditions: Store at –20°C. 37 Size Conc. Cat.# Price (Fr) 500 u 10 u/µl R7271 86.00 2,500 u R7273 301.00 Alkaline Phosphatases Product Alkaline Phosphatase, Calf Intestinal (CIAP) Size Conc. Cat.# Price (Fr) Alkaline Phosphatase, Calf Intestinal 1,000 u 1 u/µl M1821 126.00 Alkaline Phosphatase, Calf Intestinal (HC) 1,000 u 20 u/µl M2825 126.00 Available Separately CIAP Buffer Pack For Laboratory Use. Size Cat.# Price (Fr) 1.5 ml M1833 56.00 Description: Alkaline Phosphatase, Calf Intestinal (CIAP), catalyzes the hydrolysis of 5´-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. This enzyme is used to prevent recircularization and religation of linearized cloning vector DNA by removing phosphate groups from both 5´-termini and may also be used for the dephosphorylation of 5´ phosphorylated ends of DNA or RNA for subsequent labeling with [32P]ATP and T4 Polynucleotide Kinase. CIAP is active on 5´ overhangs, 5´ recessed and blunt ends. Features: • Available at High Concentration: Cat.# M2825 contains 1,000 units of CIAP at 20u/μl. • Blue/White Cloning Qualified: Promega’s blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications. • Provided with 10X Reaction Buffer: 0.5M Tris-HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM spermidine. Storage Conditions: Store at –20°C. Protocol Alkaline Phosphatase, Calf Intestinal (CIAP) Protocol Part# 9PIM182 37 Size Conc. Cat.# Price (Fr) 50 u 1–5 u/µl R6491 135.00 250 u 1–5 u/µl R6495 540.00 4-CORE® Buffer Pack Product 4-CORE® Buffer Pack (Buffers A, B, C and D), 1ml each For Laboratory Use. Description: The 4-CORE® Buffer Pack contains convenient aliquots of Promega Restriction Enzyme 10X Buffers A, B, C and D. The majority of Promega restriction enzymes have optimal activity in one of these four 10X reaction buffers. Storage Conditions: Store at –20°C. Size Cat.# Price (Fr) 4 ml R9921 43.00 88 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 91
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