PRODUCT FOCUS Purify Concentrated, Hi RNA from Cells and Tiss The ReliaPrep™ RNA Cell and Tissue Miniprep Systems offer a rapid method for purification of high-quality RNA from very small amounts of cultured cells or tissues. They also provide the option of elution in a small volume to generate highly concentrated RNA. RNA isolated from cells and tissues using the ReliaPrep™ Systems is intact, highly pure and ideally suited for applications such as RT-qPCR. • Fast, 30-minute protocol • Elute in 15μl or less to generate highly concentrated RNA • No phenol/chloroform extractions or beta-mercaptoethanol • Maximum sensitivity for downstream assays without worry of inhibition when measuring low-copy-number targets Using the ReliaPrep™ membrane-based systems, 1 × 102 to 5 × 106 0.25 to 20mg tissue can be processed per purification. High Yield and Purity of RNA from Tissue Samples A. A. 100 150 70.4 50 0 33.81 8.482 10.12 11.27 136.97 93.99 0.5 1.0 1.5 2.0 2.5 0 B. A260 A260 /A230 /A280 cultured cells or Typical RNA yields from fibrous and nonfibrous mouse tissues. RNA was isolated from 10mg samples of various mouse tissues using the ReliaPrep™ RNA Tissue Miniprep System. Yield and purity were determined by absorbance spectroscopy using a Nanodrop® 1000 instrument. enews | Fall 2013 RNA Yield (µg) Absorbance Ratio 2.2 2.13 2.18 2.1 2.1 2.1 2.2 2.15 2.21 2.12 2.11 2.08 2.16 2.06 Brain Lung Heart Kidney Liver Spleen Pancreas Brain Lung Heart Kidney Liver Spleen Pancreas Pagina 11
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